Induction of NO synthesis in macrophages by Newcastle disease virus is associated with activation of nuclear factor-{kappa}B |
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Authors: | Umansky Victor; Shatrov Vladimir A; Lehmann Volker; Schirrmacher Volker |
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Institution: | 1 The Tumor Immunology Program, Division of Cellular Immunology German Cancer Research Center Heidelberg, Germany
2 Division of Immunochemistry, German Cancer Research Center Heidelberg, Germany |
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Abstract: | Newcastle disease virus (NDV) has received much attention recentlybecause of its non-specific immune stimulating potential andits various anti-tumor activities. Here we describe that NDVinduces synthesis of NO and causes an activation of nuclearfactor-kB (NF-kB) In murine macrophages. These reactions werepart of an activation process which included also stimulationof adenosine deaminase and inhibition of 5'-nucleotidase. NDV-mediatedNO synthesis and NF-kB activation were blocked by an antioxidant(butylated hydroxyanisole), by an inhibitor of protein tyrosinekinase (genistein) and of protein kinase A (H-89), but not byan inhibitor of protein kinase C (staurosporin). These datasuggest that signalling requirements of NF-kB activation andNO production in NDV-treated macrophages are similar. |
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Keywords: | macrophages Newcastle disease virus NF-B activation" target="_blank">gif" ALT="{kappa}" BORDER="0">B activation NO induction signal transduction |
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