不同方法对羊水细胞培养成功率的影响

目的比较不同方法对羊水细胞培养成功率的影响。方法选择2019年1-10月于永州市妇幼保健院进行胎儿染色体核型检查的404例孕妇作为研究对象,采集所有孕妇的羊水细胞,分别采用原代培养法以及传代培养法进行羊水细胞培养,收获后进行低渗处理,制作吉姆萨染色玻片阅片核对。比较两种羊水细胞培养方法的成功率和失败率,对羊水标本玻片中的染色体分裂相进行计数。结果采用传代培养法的羊水细胞培养成功率明显高于原代培养法〔100.00%(404/404)比96.04%(388/404)〕,失败率明显低于原代培养法〔0(0/404)比3.96%(16/404)〕,且羊水染色体分裂相出现数(个:92.85±1.15比50.94±1.20)、可计数(个:53.78±1.22比30.30±1.25)、可分析数(个:27.34±1.06比14.55±1.05)均明显高于原代培养法,差异有统计学意义(均P<0.05)。结论采用传代培养法的羊水细胞培养成功率更高,且能够获取足够的羊水染色体核型,值得在临床检验工作中推广使用。

Effect of different methods on success rate of amniotic fluid cell culture

Objective To compare the success rate of amniotic fluid cell culture by different methods.Methods The 404 pregnant women who went to Yongzhou Maternal and Child Health Hospital from January to October 2019 to carry out fetal chromosome karyotype examination were selected as the research objects.Amniotic fluid cells were collected from all pregnant women,and the primary culture method and subculture method were used respectively.After harvest,low permeability treatment was carried out,and Gimsa stained slide was made for reading and checking.The success rates and failure rates of amniotic fluid cell culture by two methods were compared and the mitotic phases of chromosomes in amniotic fluid slides were counted.Results By subculture method,the success rate of amniotic fluid cell culture was significantly higher than that by primary culture method 100.00% (404/404) vs.96.04% (388/404)];the failure rate was significantly lower than that by primary culture method 0 (0/404) vs.3.96% (16/404)];the occurrence number (92.85±1.15 vs.50.94±1.20),countable number (53.78±1.22 vs.30.30±1.25),and analytic number (27.34±1.06 vs.14.55±1.05) of chromosome division in amniotic fluid were significantly higher than those by primary culture (all P < 0.05).Conclusion The success rate of amniotic fluid cell culture by subculture is higher and enough amniotic fluid chromosome karyotypes can be obtained,which is worth popularizing in clinical test.