人肝硬化组织中DMBT1的表达与卵圆细胞增生的关系

目的 探讨DMBTl在人类肝硬化组织中的表达及其与卵圆细胞增殖的关系.方法 对22例人肝硬化标本进行CK19、Hep和DMBT1免疫组化染色,用激光显微切割方法研究DMBT1在增生的胆小(PBD)中的缺失(LOH),同时对其中5例标本进行电镜观察·以10例正常肝脏作对照.结果 光镜下,所有肝硬化组织中均可找到少量卵圆细胞,其数量与炎症程度成正相关,电镜下证实卵圆细胞位于PBD中.DMBT1在卵圆中细胞也有表达,其在PBD中的阳性率为45.5%.LOH分析发现DMBT1在PBD中的发生率为45.5%,与周围肝细胞相比有显著性差异(P<0.05).而正常肝组织中未找到双向表达的卵圆细胞,仅少量大胆管有DMBTl的表达.结论 人肝硬化组织中存在双向分化的卵圆细胞,位于PBD中,DMBT1在此类细胞中高表达,DMBT1 LOH较特异的出现在PBD中,并与蛋白表达呈现相对一致性,说明DMBT1可能参与卵圆细胞的分化增殖,在肝硬化的发生与发展中可能起到调控作用.

Expression of DMBTI in liver cirrhosis and its relation to oval cell proliferation

Objective To investigate the expression of DMBT1 in liver cirrhosis and its relation to oval cell proliferation.Methods Immunostaining was carried out for CK19, Hep and DMBT1 in 22 liver cirrhotic samples.The homozygous deletion of DMBT1 in proliferated bile ductules (PBD) was investigated by laser capture microdissection (LCM).Meanwhile, the samples from 5 cases were ex-amined with transmission electron microscopy.Ten normal liver tissues were used as controls.Results Microscopically, oval cells could be found in all cirrhotic cases which could coexpress CK19 and Hep and its number was found to increase significantly with the progression of inflammation.Electron mi-croscope identified the presence of oval cells in PBD.DMBT1 could express in these cells too and its positive rate in PBD was 45.5 %.The homozygous deletion of DMBT1 was found in 45.5% PBD.In normal liver tissues, PBD and oval cells were not found.DMBT1 expression was only detectable in few large bile ducts.Conclusion There are bipotent oval cells with high expression of DMBT1 in liver cirrhosis hiding in PBD and DMBT1 can be specifically deleted in PBD, suggesting that DMBT1 may be related to the proliferation of oval cells and may participate in the development of liver cirrhosis.