| 肝脏及肢体缺血预处理抗大鼠肝缺血再灌注损伤的实验研究 |
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| 引用本文： | 陈闯|李文美|蒋厚文|郝立俊. 肝脏及肢体缺血预处理抗大鼠肝缺血再灌注损伤的实验研究[J]. 中国普通外科杂志, 2010, 19(1): 23-27 |
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| 作者姓名： | 陈闯|李文美|蒋厚文|郝立俊 |
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| 作者单位： | 徐州医学院附属淮安医院普通外科;徐州医学院附属医院普通外科; |
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| 基金项目： | Reperfusion Injury； Liver/blood supply; Ischemic Preconditioning； Rats |
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| 摘 要： | 目的研究肝脏缺血预处理(经典缺血预处理IPC)的第一保护窗(FW)与肢体缺血预处理(远端缺血预处理RPC)的第二保护窗(SW)及两者联合应用对大鼠肝脏缺血再灌注(I/R)损伤的保护作用及可能机制。方法大鼠随机分成5组:I/R组不行预处理;IPC组以肝缺血5 min行预处理;RPC组以双后肢缺血5 min,反复3次行预处理;RPC+IPC组先行RPC,24 h后行IPC作预处理;S组仅行开腹,不行其他处理。3个预处理组及I/R组均行肝缺血1 h再灌注3 h。取血用于血清谷丙转氨酶(ALT)与血清谷草转氨酶(AST)检测。切取肝组织用于测定肿瘤坏死因子α(TNF-α)和热休克蛋白70(HSP70)的表达、湿干比(W/D)及观察显微、超微结构的变化。结果与I/R组比较,IPC组,RPC组及RPC+IPC组ALT,AST,W/D及TNF-α阳性表达均明显降低(P0.01),HSP70表达量明显增加(P0.01),肝脏的显微及超微结构损伤减轻;IPC,RPC,RPC+IPC组3组间各项指标差异无统计学意义(P0.05)。结论IPC的FW,RPC的SW及两者联合应用对大鼠肝脏I/R损伤均有明显的保护作用,三者在保护强度上无明显差异。其机制可能与抑制TNF-α的产生、诱导保护性蛋白HSP70的表达、减轻肝脏水肿、改善肝组织微循环有关。
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| 关 键 词： | 再灌注损伤； 肝/血液供应； 缺血预处理； 大鼠 |
| 收稿时间： | 2009-07-31 |
| 修稿时间： | 2009-10-26 |
Study on the protective effect of liver and limbs ischemic preconditioning against liver ischemia/reperfusion injury in the rat |
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| CHEN Chuang|LI Wenmei|JIANG Houwen|HAO Lijun. Study on the protective effect of liver and limbs ischemic preconditioning against liver ischemia/reperfusion injury in the rat[J]. Chinese Journal of General Surgery, 2010, 19(1): 23-27 |
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| Authors: | CHEN Chuang|LI Wenmei|JIANG Houwen|HAO Lijun |
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| Affiliation: | CHEN Chuang1,LI Wenmei2,JIANG Houwen1,HAO Lijun1(1.Department of General Surgery,Affiliated Huaian Hospital,Xuzhou Medical College,Huaian,Jiangsu 223002,China,2.Department of General Surgery,Affiliated Hospital,Xuzhou,Jiangsu 221002,China) |
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| Abstract: | Objective To investigate the protective effect of the first window(FW)of liver ischemic preconditioning(IPC),the second window(SW) of remote(leg) ischemic preconditioning(RPC) and conbined applications of liver and lges IPC to against liver ischemia/reperfusion(I/R) injury in the rat,and to investigate the mechanism of the protection.Methods Rats were randomly divided into five groups(n=8 each):(1) Sham group(S group),rats without IPC,(2) Rats with 5 min IPC(IPC group);(3) Rat wiht both liver and lower limb... |
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| Keywords: | Reperfusion Injury Liver/blood supply Ischemic Preconditioning Rats |
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