增殖细胞核抗原在增生性瘢痕组织中的表达与分布

目的　研究增殖细胞核抗原 (PCNA)在增生性瘢痕组织中的表达水平和分布特点 ,探讨该组织中细胞增殖的特征。方法　利用PCNA单克隆抗体 ,对 12例增生性瘢痕、8例成熟瘢痕及其配对的正常皮肤 ( 2 0例 )进行免疫组织化学染色 (SABC法 )。结果　在增生性瘢痕组织中 ,PCNA标记率 ( 4 5 4± 12 3 )明显高于正常皮肤 ( 13 5± 4 1)和成熟瘢痕 ( 17 4± 6 2 ) ,差异有非常显著意义(P <0 0 1) ;而后两者间差异无显著意义。其中表皮角朊细胞和真皮成纤维细胞的细胞核内可见阳性颗粒。结论　增生性瘢痕组织中表皮角朊细胞和真皮成纤维细胞均增殖活跃 ,两者与细胞外基质过度沉积均有密切关系 ;PCNA是判断细胞增殖状态的良好指标 ,是诊断增生性瘢痕的客观依据

The study of expression and distribution of PCNA in hypertrophic scar tissue

OBJECTIVE: To analyze the expression and the distribution of proliferation cell nuclear antigen (PCNA) in hypertrophic scar for identifying its certain characteristics during the fibroblast proliferation. METHODS: Bioptic samples were taken form 12 cases of hypertrophic scar, 8 cases of matured scars and 20 cases of controlled normal skin. Each sample was immunohistochemically stained and examined with monoclonal antibodyPCNA(pc10)] technique. The level of PCNA expression was decided for evaluation of the fibroblast proliferation. RESULTS: The level of PCNA in the hypertrophic scar(45.4 +/- 12.3) was significantly higher than mature scar(17.4 +/- 6.2), also normal skin(13.5 +/- 4.1, P < 0.01). But, there was no significant deference between the matured scar and the normal skin. The PCNA intracellular particles may sometimes be bound in the keratinocytes and the fibroblasts of some connective tissue. CONCLUSION: The fibroblast proliferation in the hypertrophic scar has kept a high level. During the process of hypertrophic scar formation it may play an important role.