增强型体外反搏对高血脂猪阴茎海绵体组织结构影响

目的:观察增强型体外反搏对高血脂猪阴茎海绵体组织结构的改变。方法:建立猪高胆固醇血症模型同窝生乳猪12只,随机均分为对照组(n=6)和反搏组(n=6),反搏组从2.5月龄完成36h的EECP方案,采集组织标本连续切片HE染色后置于光学显微镜高倍视野下观察,观察内容为内皮细胞形态、纤维及平滑肌增生情况,并根据病变轻重排序分别赋予1～12分值。结果:反搏组阴茎海绵体光镜下病理等级分为(1、2、3、5、7、8),对照组阴茎海绵体光镜下病理等级分为(4、6、9、10、11、12),非参数检验Mann-Whitney Test,P<0.05有统计意义。病理观察时反搏组及对照组比较可见:①内皮细胞排列对照组较反搏组紊乱,肿胀及脱落的数量较多;②纤维增生两组均可见,多伴有粘液样变,两组比较差别不明显;③两组均见平滑肌增生,多伴粘液样变,对照组平滑肌增生程度较反搏组明显。结论:增强型体外反搏可以改善或延缓高脂血症对阴茎海绵体的病理损害。

Effect of EECP on structure of Penile Corpus Cavernosum in Porcine with Hypercholesterolemia

Objective： To explore the effect of long-term enhanced external counterpulsation （EECP） on structure of penile corpus cavernosum in porcine with hypercholesterolemia. Methods： Twelve male infant pigs were randomly divided into 2 groups according to treatment： the hypercholesterolemic control group （n = 6） and the hypercholesterolemic ＋ EECP group（n=6）. After having performed EECP for 36 hours on the hypercholesterolemic ＋ EECP group（n=6） , we collected the sections from penile corpus cavernosum of every pigs and sorted the sections in sequence according to their morphology of endothelial cells , hyperplasia of fibrobtasts and smooth muscle cells. Results： The pathological sequence of hypercholesterolemic ＋EECP group was obviously lower compared with that of the hypercholesterolemic control group （P〈 0. 05）. Con- elusion： Long-term EECP may protect penile, corpus cavernosum from the damage resulting from hypercholes- terolemias.