单侧隐睾大鼠生殖股神经在对侧睾丸损害中的作用机制研究

目的 :探讨单侧隐睾大鼠模型生殖股神经在对侧睾丸损害中的作用机制。　方法 :建立单侧隐睾大鼠模型 (2 1d龄 ) ,切断该侧生殖股神经 ,12 0d后观察对侧睾丸的生精细胞凋亡变化及组织乳酸含量变化。　结果 :切断生殖股神经后 ,对侧睾丸生精细胞凋亡为 (5 .76± 0 .76 ) % ,与对照组 (17.2 8± 1.36 ) %相比明显减少 (P <0 .0 5 ) ;乳酸含量也由 (2 .19± 0 .2 4 )mmol/L下降为 (1.70± 0 .31)mmol/L(P <0 .0 5 ) ,且乳酸含量与细胞凋亡呈正相关(P <0 .0 5 )。　结论 :单侧隐睾症对侧睾丸损伤可能与其神经传导反射性血流减少引起生精细胞凋亡有关

Investigation of the Mechanism of Genitofemoral Nerve in the Contralateral Testes Damage of Unilateral Cryptorchid Rats

OBJECTIVES: To investigate the mechanism of genitofemoral nerve in the contralateral testes damage of unilateral cryptorchid rats. METHODS: After unilateral cryptorchid rat model (21-day) was made and the ipsilateral genitofemoral nerve was divided, the germ cells apoptosis and the concentrations of lactate of contralateral testes were detected and compared after 120 days. RESULTS: After the ipsilateral genitofemoral nerve was divided, the germ cells apoptosis and the lactate levels of the contralateral testes were all decreased from (17.28 +/- 1.36)% to (5.76 +/- 0.76)% and (2.19 +/- 0.24) mmol/L to (1.70 +/- 0.31) mmol/L, respectively. CONCLUSIONS: The damage pathway of the contralateral testes of unilateral cryptorchidism probably related to the apoptosis of the germ cells which caused by the reflex decrease of the contralateral testicular blood flow via the transmissions of genitofemoral nerve.