全氟辛烷磺酸和全氟辛酸对秀丽隐杆线虫的生殖毒性

目的：探讨全氟辛烷磺酸（PFOS）和全氟辛酸（PFOA）对秀丽隐杆线虫的生殖毒性，寻找评价PFOS和PFOA生殖毒性的替代模型。方法：PFOS和PFOA分别设高、中、低3个染毒剂量（0.1、0.01、0.001 mmol/L）组和空白对照组，同步化后L4期幼虫在24孔板里染毒24 h，L1期幼虫染毒48 h，测定后代数目和世代时间。结果：L4期幼虫染毒24 h后，与对照组比较，PFOS高、中剂量组后代数目差异具有统计学意义（P<0.05），PFOA的3个剂量组后代数目差异均有统计学意义（P<0.05）；PFOS和PFOA各剂量组世代时间差异均无统计学意义（P>0.05）。L1期幼虫染毒48 h后，与对照组比较，PFOS和PFOA高、中剂量组后代数目差异具有统计学意义（P<0.05）；PFOS 3个剂量组的世代时间差异均具有统计学意义（P均<0.05）。结论：后代数目是PFOS和PFOA生殖毒性的敏感指标，L1期幼虫的世代时间对PFOS暴露比L4期幼虫更敏感，但PFOA不影响线虫的世代时间。线虫可能成为评价PFOS和PFOA生殖毒性的替代模型。

Reproductive toxicity of perfluorooctane sulfonate and perfluorooctanoic acid on Caenorhabditis elegans

OBJECTIVE: Caenorhabditis elegans were used to investigate the induction of reproductive toxicity of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA),as an alternative models for evaluating reproductive toxicity. METHODS: Three PFOS/PFOA dose (0.1,0.01,0.001 mmol/L) groups and a control group were organized. After synchronization,L4 larvae were exposed for 24 h in 24-well plates,the L1 larvae were exposed for 48 h. The brood size and the generation time of Caenorhabditis elegans were evaluated to determine the induction of reproductive toxicity by PFOS and PFOA. RESULTS: Compared with the control,the high and the medium dose groups with exposure for 24 h showed significant difference in the brood size (P<0.05),and the three PFOA exposure dose groups showed significant difference among them (P<0.05). Generation time showed no significant difference among the exposed and the control groups. Compared with the control group,the high and medium dose groups with exposure for 48 h showed significant difference in the brood size (P<0.05) and the three exposed groups showed significant difference in generation time (P<0.05). CONCLUSION: The results suggested that brood size of Caenorhabditis elegans was a sensitive indicator for evaluating reproductive toxicity of PFOS and PFOA. In addition,generation time measurements was more sensitive using L1 larvae than that of L4 larvae.