糖尿病患者血清一氧化氮、丙二醛变化的意义

目的探讨血清一氧化氮 (NO)、丙二醛 (MDA)在 2型糖尿病 (DM )患者中的变化及临床意义。方法用酶标法及硫代巴比妥酸比色法分别测定正常对照者 2 4名、2型糖尿病无并发症患者 2 2例、并糖尿病肾病患者 30例的血清NO、MDA水平。结果DM患者血清MDA水平明显高于对照组 (P <0 .0 1) ,其中糖尿病肾病组增高更为明显。糖尿病肾病组NO水平有所降低 ,但差异不显著(P >0 .0 5 )。病程 6年以上的糖尿病患者血清NO水平明显降低 (P <0 .0 1)。结论糖尿病患者体内存在氧化系统的异常 ,氧自由基与糖尿病肾病的发生有关 ;NO对其发生、发展亦可能起一定作用。

Blood Serum Nitric Oxide,Malondialdehyde in Patients with Diabetes Mellitus and its Clinical Significance

Objective To explore the clinical significance of blood serum nitric oxide (NO) and malondialdehyde (MDA) in patients suffering from diabetes mellitus type 2 (T2DM). Methods The concentrations of blood serum NO and MDA were measured with the enzyme immunoassay and TBA colorimetric methods. The samples were collected from 22 T2DM patients without complications, 30 T2DM patients with diabetic nephropathy and 24 healthy individuals as controls. Results The mean blood serum concentration of MDA in diabetes mellitus (DM) patients, especially in diabetic nephropathy patients was significantly higher than that in healthy control group(P<0.01). The concentration of NO in diabetic nephropathy patients was lower, but it was not significant (P>0.05). And it was significantly lower in DM patients whose course of DM was over 6 years (P<0.01).Conclusion Oxygen free radical is associated with development of diabetic nephropathy.