Dendritic outgrowth of myenteric plexus neurons in primary culture. |
| |
Authors: | M W Mulholland G Romanchuk K Flowe |
| |
Institution: | Department of Surgery, University of Michigan, Ann Arbor 48109-0331. |
| |
Abstract: | Myenteric plexus neurons derived from neonatal guinea pigs, when exposed to serum, demonstrated a characteristic pattern of growth, including a proliferating outgrowth zone of glial cells, peripheral extension of dendritic processes, and progressive dendritic growth. Serum effects upon dendritic growth, measured morphometrically, was strongly dose- and temporally dependent. Dendritic density was increased 10-fold (120 hr) by the addition of 6% serum, while mean dendritic length was increased 3-fold. Development of cholinergic function was reflected by release of 3H]ACh in response to cholecystokinin octapeptide, vasoactive intestinal peptide, substance P, and calcitonin gene-related peptide (10(-10) and 10(-8) M). |
| |
Keywords: | |
|
|