人肺癌细胞转染野生型P53基因对生长的影响

目的：研究转染野生型Ｐ５３基因对人肺癌细胞的生长影响作用。方法：将带有野生型Ｐ５３基因的ｐＣＥＰ４质粒，通过Ｌｉｐｏｆｅｃｔｉｎ转染肺癌细胞，用四甲基偶氮唑（ＭＴＴ）法观察细胞的生长情况。结果：转染野生型Ｐ５３基因，可使肺腺癌、肺鳞癌和小细胞肺癌细胞的生长明显受到抑制。结论：野生型Ｐ５３基因有效抑制肺癌细胞的生长，可作为肺癌基因治疗的一种手段。

STUDY ON EFFECT OF TRANSFECTION WITH WILD-TYPE P53 GENE ON GROWT H OF LUNG CARCINOMA CELLS

Objective:To observe the effecti on the growth of lung carcinoma cells by transfection with wild type P 53 (wt-P 53 )gene on growth Methods:The lung carcinoma cells were transfected by the Plasmids pCEP4 recombined with wild type P 53 gene at lipofectin mediated The growing tests of the lung carcinoma cells were studied by monotetrazolium(MTT) analysis Results:The growth of the lung adenocarcinoma cells,lung squamous cell carcinoma cells as well as small cell lung carcinoma cells were observably suppressed after transfection with the wt-P 53 gene Conclusion:The wt P 53 gene effectively suppressed the growth of lung carcinoma cells,and this is a way for the gene therapy of lung carcinoma