Isolation of vascular smooth muscle cells from a single murine aorta |
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Authors: | Lynn Ray Jenna Leach Rebecca Herbert Jean-Marc Benson Mark |
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Institution: | (1) Department of Internal Medicine, Cardiovascular Research Center, University of Michigan Medical School, Ann Arbor, MI, USA;(2) Cardiovascular/Thrombosis Research Department, Sanofi-Synthelabo, Toulouse Cedex, France |
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Abstract: | The vascular smooth muscle cell plays a significant role in many important cardiovascular disorders, and smooth muscle biology is therefore important to cardiovascular research. The mouse is critical to basic cardiovascular research, largely because techniques for genetic manipulation are more fully developed in the mouse than in any othermammalian species. We describe here a technique for isolating smooth muscle cells from a single mouse aorta. This technique is particularly useful when material is limiting, as is frequently the case when genetically modified animals are being characterized. |
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Keywords: | Smooth muscle Tissue culture Transgenic Vascular biology |
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