| 应用不同微生物源追踪方法追踪水库中粪便污染来源 |
| |
| 引用本文： | 顾玲,丁震,汪华,陈晓东,李伟伟,叶珣,刘晓阳,张磊,林龙,胡晓抒.应用不同微生物源追踪方法追踪水库中粪便污染来源[J].中国卫生检验杂志,2010(2). |
| |
| 作者姓名： | 顾玲 丁震 汪华 陈晓东 李伟伟 叶珣 刘晓阳 张磊 林龙 胡晓抒 |
| |
| 作者单位： | 江苏省疾病预防控制中心;南京医科大学公共卫生学院;江苏省盱眙县疾病预防控制中心;江苏省盱眙县桂五镇中心卫生院; |
| |
| 摘 要： | 目的:用两种不同的微生物源追踪(Microbial source tracking,MST)方法对江苏省盱眙县桂五水库粪便污染来源进行追踪。方法:于春、夏、秋、冬4季分别采集水库周边已知来源粪便标本,分离大肠埃希菌并作为指示菌,分别建立已知源指示菌细菌基因组重复序列PCR(repetitive Extragenic Palindromic-Polymerase chain reaction,rep-PCR)特征指纹库和已知源指示菌抗生素敏感性(Antibiotic Resistance Analysis,ARA)数据库,并分别用统计分析及试验设计软件(JMP7.0软件)和基因聚类分析软件(Bionumerics 4.0软件)计算平均正确归类率(Average Rate of Correct Classification,ARCC),以检验各自的宿主来源区分效果。同期采集水样,膜过滤法分离并确认指示菌,分别进行rep-PCR扩增和ARA试验,并分别与已知源指示菌rep-PCR特征指纹库和ARA数据库进行统计学比对,判断桂五水库中粪便污染来源。结果:将已知源指示菌rep-PCR特征指纹库和ARA数据库分别分为2、...
|
| 关 键 词： | 微生物源追踪 大肠埃希菌 细菌基因组重复序列PCR 抗生素敏感性试验 平均正确归类率 粪便污染 |
Tracking sources of fecal pollution from reservoir by using two different microbial source tracking methods |
| |
| GU Ling,DING Zhen,WANG Hua,CHEN Xiao-dong,LI Wei-wei,YE Xun,LIU Xiao-yang,ZHANG Lei,LIN Long,HU Xiao-shu.Tracking sources of fecal pollution from reservoir by using two different microbial source tracking methods[J].Chinses Journal of Health Laboratory Technology,2010(2). |
| |
| Authors: | GU Ling DING Zhen WANG Hua CHEN Xiao-dong LI Wei-wei YE Xun LIU Xiao-yang ZHANG Lei LIN Long HU Xiao-shu |
| |
| Institution: | GU Ling1,DING Zhen1,WANG Hua1,CHEN Xiao-dong1,LI Wei-wei2,YE Xun1,LIU Xiao-yang3,ZHANG Lei3,LIN Long4,HU Xiao-shu1(1.Jiangsu Center for Disease Control , Prevention,Nanjing 210009,China,2.Nanjing Medical University,Nanjing 210029,3.Xuyu County Center for Disease , Control,Xuyi 211700,4.Xuyu County Guiwu Health Care Station,China) |
| |
| Abstract: | Objective:To track the fecal pollution sources from Guiwu reservoir at Xuyu,Jiangsu Province by using two different microbial source tracking methods(MST).Methods:Fresh fecal samples from human and animals(poultry,livestock,dogs,and wild animals) were collected throughout villages around Guiwu reservoir in Spring,Summer,Autumn and Winter of a year.Escherichia coli were isolated and identified by API20E as indicator.A known-sources fingerprinting library and a known-source antibiotic resistance library conta... |
| |
| Keywords: | Microbial source tracking Escherichia coli Repetitive extragenic palindromic-polymerase chain reaction Antibiotic resistance Analysis The average rates of correct classification Fecal pollution |
| 本文献已被 CNKI 等数据库收录！ |
|
