Variation in properties of SV40-transformed simian cell lines detected by superinfection with SV40 and human adenoviruses |
| |
| Authors: | J S Butel L S Richardson J L Melnick |
| |
| Affiliation: | 1. Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt;2. Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21568, Egypt;1. Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control, College of Animal Science and Technology, Tarim University, Alar, Xinjiang 843300, China;2. Lab for Sustainable Antimicrobials, Department of Pharmacy, Sichuan Agricultural University, Chengdu, Sichuan 610000, China;3. Instrumental Analysis Center, Tarim University, Alar, Xinjiang 843300, China;4. National Reference Laboratory of Veterinary Drug Residues (HZAU) and MARA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei 430070, China;5. Medicine and Infectious Diseases Department, Faculty of Veterinary Medicine, University of Sadat City, 32897, Egypt;6. Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, QG, Egypt;7. Engineering Center of Innovative Veterinary Drugs, Center for Veterinary Drug Research and Evaluation, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing Jiangsu 210095, China;1. Institute of Pharmaceutical Technology and Biopharmacy, University Muenster, Corrensstraße 48, 48149 Muenster, Germany;2. Bayer AG, Process Technologies, Chempark, Building E41, 51368 Leverkusen, Germany;3. Bayer AG, Pharmaceuticals, Drug Product Development, Friedrich-Ebert-Straße 217-333, 42117 Wuppertal, Germany |
| |
| Abstract: | Four different simian cell lines transformed by complete or defective SV40 were found to exhibit a variation in susceptibility to superinfection by SV40; two lines were susceptible to complete SV40 virions, one was resistant to complete virus but susceptible to SV40 DNA, and the fourth was markedly resistant to superinfection by viral DNA as well as complete virions.All four cell lines supported the replication of PARA-adenoviruses. When the transformed cell lines were superinfected by a cytoplasmic variant of PARA, the cells continued to exhibit SV40 T antigen in an intranuclear location. One of the four cell lines failed to support the growth of human adenoviruses, while the other three did so efficiently.It can be concluded that neither immunity to superinfection by the homologous virus nor the expression of the SV40 function which complements the growth of human adenoviruses is essential for maintenance of the transformed state in simian cells. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
