Adenosine suppresses GABAA receptor-mediated responses in rat sacral dorsal commissural neurons |
| |
Authors: | Li Hui Wu Le Li Yun-Qing |
| |
Affiliation: | Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, No. 17, W. Chang-le Road, Xi'an 710032, PR China. |
| |
Abstract: | The modulatory effect of adenosine on gamma-aminobutyric acid (GABA)-activated whole-cell currents were investigated in the neurons acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) using the nystatin perforated patch recording configuration under the voltage-clamp conditions. The results showed that: (1) GABA acted on GABAA receptor and elicited inward Cl- currents (IGABA) at a holding potential (VH) of -40 mV; (2) adenosine suppressed GABA-induced Cl- current without affecting the reversal potential of IGABA and the apparent affinity of GABA to its receptor; (3) N6-cyclohexyladenosine mimicked the suppression effect of adenosine on IGABA, whereas 8-cyclopentyl-1,3-dipropylxanthine blocked the suppression effect of adenosine; (4) adenosine fails to suppress IGABA on the neurons that were pretreated with bisindolylmaleimide I (BIM), while after pretreatment with H-89, the inhibitory effect of adenosine on IGABA were not affected; (5) the suppression effect of adenosine on IGABA remained in the presence of BAPTA-AM. The present results indicate that the suppression of adenosine on IGABA is mediated by adenosine A1 receptor and through a Ca2+-independent protein kinase C transduction pathway, and that the interactions between adenosine and GABA might participate in the modulation of nociceptive information transmission at the SDCN. |
| |
Keywords: | Sacral dorsal commissural nucleus GABA Protein kinase C Nociception Nystatin perforated patch clamp |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|