| 青蒿素对血管平滑肌细胞增殖的影响 |
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| 引用本文： | 周志明,冯义柏.青蒿素对血管平滑肌细胞增殖的影响[J].心肺血管病杂志,2005,24(2):109-111. |
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| 作者姓名： | 周志明 冯义柏 |
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| 作者单位： | 430030,武汉,华中科技大学同济医学院附属协和医院心内科 |
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| 摘 要： | 目的 :观察青蒿素 (artemisinin ,Art)对体外培养大鼠主动脉平滑肌细胞 (VSMC)的增殖、DNA合成及细胞周期的影响 ,探讨作用机制。方法 :体外培养大鼠主动脉VSMC ,分为不同浓度的Art组及对照组。观察细胞生长曲线 ;四甲基偶氮唑盐微量酶反应 (MTT)法检测细胞活性 ;3H 胸腺嘧啶脱氧核苷(3H- TdR)掺入法观察其对DNA合成的影响 ;通过HE染色、DNA梯带检测和流式细胞术观察细胞的凋亡并测定细胞周期。结果 :与对照组相比 ,各浓度Art组VSMC计数不同程度减少 ,3H- TdR掺入率降低 ,细胞增殖受抑制 ,VSMC凋亡增加 ,并呈浓度依赖性 ;Art诱导细胞凋亡 ;使细胞周期阻滞于G0 / G1 期。结论 :Art对体外培养VSMC的增殖有抑制作用 ,可能通过抑制细胞DNA合成 ,诱导细胞凋亡及干扰细胞周期等途径发挥作用
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| 关 键 词： | 青蒿素 平滑肌细胞 脱氧核糖核酸 凋亡 细胞周期 |
| 修稿时间： | 2004年8月14日 |
Effect of artemisinin on the proliferation of cultured vascular smooth muscle cell |
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| ZHOU Zhiming,FENG Yibai.Effect of artemisinin on the proliferation of cultured vascular smooth muscle cell[J].Journal of Cardiovascular and Pulmonary Diseases,2005,24(2):109-111. |
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| Authors: | ZHOU Zhiming FENG Yibai |
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| Institution: | ZHOU Zhiming,FENG Yibai Department of Cardiology,Union Hospital,Huazhong University of science and technology,Wuhan 430030,China |
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| Abstract: | Objective:To observe the effects of Artemisinin (Art) on the proliferation of cultured rats vascular smooth muscle cell (VSMC) in vitro and analyses the mechanism.Method:VSMC was isolated from rat's aorta and cultured with different concentration of Art. Growth curve of VSMC was recorded, DNA replication were evaluated by 3H]-TdR method. Flow metometry was used to analyze cell apoptosis and cell cycles.Result:Comparing to the control group, VSMC proliferation of Art interfering groups were inhibited, and 3H]-TdR intermingle rate were decreased as well as cell apoptosis were induced. The cell cycle were blocked at G 0/G 1 phase by Art in a dose dependent.Conclusion:Art inhibits VSMC proliferation by disturbing DNA replication, cell apoptosis and blocking cell cycle at G 0/G 1 phase. |
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| Keywords: | Artemisinin Vascular smooth muscle cell Deoxyribonucleic acid Apoptosis Cell cycle |
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