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| 莪术提取物对PDGF诱导的肝星状细胞内Ca2+和PI3-K的影响 | |
| 引用本文： | 熊振芳,朱清静,杨玲,张赤志.莪术提取物对PDGF诱导的肝星状细胞内Ca2+和PI3-K的影响[J].中西医结合肝病杂志,2007,17(6):358-360. |
| 作者姓名： | 熊振芳 朱清静 杨玲 张赤志 |
| 作者单位： | 1. 湖北中医学院,湖北 武汉,430061 2. 武汉市传染病医院 3. 华中科技大学同济医学院附属协和医院 |
| 基金项目： | 湖北省教育厅科研项目;湖北省自然科学基金 |
| 摘 要： | 目的:探讨莪术提取物药物血清对PDGF诱导的肝星状细胞(HSC)内Ca2 、PI3-K的影响。方法:制备药物血清,不同浓度的莪术提取物药物血清温育肝星状细胞24小时后,PDGF-BB(10ng/ml)刺激24小时,再加入上述浓度的莪术提取物药物血清,3小时后,又加入PDGF-BB(10ng/ml)作用5分钟,然后收集细胞。采用钙指示剂Fura-2/Am测定细胞内Ca2 ,免疫印迹化学发光法检测PI3-K的表达。结果:不同浓度的莪术提取物药物血清与同浓度的生理盐水药物血清比较,能显著抑制PDGF诱导的胞内Ca2 浓度升高(P<0.01)。经10%莪术提取物药物血清处理的HSC,其PDGF诱导的PI3-K表达水平较10%生理盐水药物血清组显著降低(P<0.01)。结论:莪术提取物药物血清能抑制PDGF诱导的肝星状细胞内Ca2 内流及PI3-K的表达。 |
| 关 键 词： | 莪术提取物/药效学 肝纤维化 肝星状细胞 血小板源生长因子 |
| 收稿时间： | 2007-10-26 |
| 修稿时间： | 2007年10月26 |
Effect of Rhizoma Curcumaein on Ca2+ and PI3-K of HSC induced by PDGF |
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| XIANG Zhen-fang, ZHU Qing-jing, YANG Ling, et al China.Effect of Rhizoma Curcumaein on Ca2+ and PI3-K of HSC induced by PDGF[J].Chinese Journal of Integrated Traditonal and Western Medicine on Liver Diseases,2007,17(6):358-360. | |
| Authors: | XIANG Zhen-fang ZHU Qing-jing YANG Ling China |
| Abstract: | Objective:To investigate the role of Rhizoma Curcumaein extract serum(ERCS) on the changes of Ca2 and PI3-K inducing by PDGF.Methods:HSC were treated with ERCS for 24 hours,and stimulated with PDGF-BB for 24 hours,and added the same concentration of ERCS,3 hours later,stimulated with PDGF-BB for 5 minutes,then collected the cells.Intracellular Ca2 concentration was measured with calcium probe.PI3-K was detected with western blotting enhenced chemiluminescent(ECL) method.Results:ERCS could inhibit the increasing of intacellular calcium concentration induced by PDGF-BB(P<0.01).Treatment of HSC with ERCS resulted in an inhibition of the PDGF-BBinduced expression of PI3-K(P<0.01).Conclusion:ERCS can inhibti intracellular signal transudation of PDGF in HSC to block the biological effect of PDGF. |
| Keywords: | rhizoma curcumaein/pharmacodyamics liver fibrosis HSC PDGF |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |