大鼠脑胶质瘤伽玛刀治疗后细胞增殖和凋亡的变化

目的：探讨大鼠脑胶质瘤模型伽玛刀治疗后细胞凋亡水平和增殖活性时程变化，方法：将接种C6胶质瘤细胞的120只大鼠分为空白对照组和伽玛刀治疗组，于治疗后不同时程行流式细胞学检测和增殖细胞核抗原（PCNA）免疫组织化学研究，观察其细胞凋亡和增殖情况，结果：伽玛刀治疗后肿瘤细胞凋亡率较对照组显著提高（P＜0．01），并于伽玛刀照射后48h达到高峰。随时间逐渐下降；治疗组肿瘤内PCNA阳性细胞数量较对照组显著下降（P＜0．05），随时间PCNA阳性细胞数量逐渐减少，结论：大鼠脑胶质瘤伽玛刀治疗后除细胞坏死外，肿瘤细胞增殖水平下降和细胞凋亡也是伽玛刀治疗胶质瘤的机理之一。

The changes of the rat glioma cell apoptosis and proliferation after gamma knife treatment

Objective\ To evaluate the changes of apoptosis level and proliferated activity of the rat glioma model before and after gamma knife treatment. Methods\ 50 rats planted with C6 glioma cell were treated with gamma knife,at different time before and after irradiation,to observe the apoptosis of the glioma cell by flow cytometry and the proliferated activity by PCNA immunohistochemistry. Results\ The apoptosis rate was increased significiently after gamma knife treatment( P <0 01),and reached peak at the 48 th h; The positive cells number of PCNA the treatment group is significantly less than the control group,and continued to decrease with the following time point. Conclusion\ In addition to the necrosis,the decrease of the proliferation level and the increasing of the apoptosis rate may also the factors which inhibit the tumor growing after gamma knife irradition.