Cryopreserved buffy-coat-derived platelets reconstituted in platelet additive solution: A safe and available product with sufficient haemostatic effectiveness |
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| Authors: | Hana Lejdarova Rita Pacasova Lenka Tesarova Irena Koutna Nadezda Polokova Simona Michlickova Martin Dolecek |
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| Affiliation: | 1. Department of Transfusion and Tissue Medicine, University Hospital Brno, Jihlavska 20, 625 00, Brno, Czech Republic;2. Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic;3. International Clinical Research Centre, St. Anne’s University Hospital Brno, Pekarska 53, 656 91, Brno, Czech Republic;4. Clinic of Anaesthesiology, Resuscitation and Intensive Medicine, University Hospital Brno, Jihlavska 20, 625 00, Brno, Czech Republic;1. Ege University Medical Faculty, Division of Endocrinology and Metabolism Disorders, Izmir, 35100, Turkey;2. Ege University Medical Faculty, Division of Hematology, Izmir, Turkey;3. Ege University Medical Faculty, Division of Clinical Biochemistry, Izmir, Turkey;4. Adnan Menderes University Medical Faculty, Division of Hematology, Aydin, Turkey;1. Department of Anaesthesia, Galway University Hospitals, Galway, Ireland;2. Department of Anaesthesia, Mayo University Hospital, Mayo, Ireland;3. Department of Anaesthesia, Letterkenny University Hospital, Donegal, Ireland;4. Department of Anaesthesia, Sligo University Hospital, Sligo, Ireland;5. School of Medicine, National University of Ireland, Galway, Ireland;6. School of Medicine, National University of IreLand, Galway, Ireland;7. Department of Anaesthesia, Galway University Hospitals, Galway, Ireland;1. Department of Laboratory Medicine, Tama-Hokubu Medical Center, Tokyo Metropolitan Health and Medical Treatment Corporation, Tokyo, Japan;2. Department of Hematology, Tama-Hokubu Medical Center, Tokyo Metropolitan Health and Medical Treatment Corporation, Tokyo, Japan;3. Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Tokyo, Japan;4. Clinical Research Support Center, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, Japan;1. Department of Pediatrics, Hematology and Oncology, Medical University of Warsaw, Zwirki i Wigury str. 63A, 02- 091 Warsaw, Poland;2. Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Zwirki i Wigury str. 63A, 02- 091 Warsaw, Poland;3. Institute of Hematology and Transfusion Medicine, Warsaw, Poland Indiry Gandhi str. 14, 02-776 Warsaw, Poland;4. Stem Cell Bank, Central Clinical Hospital Warsaw Medical University, Poland, Zwirki i Wigury str. 63A, 02- 091 Warsaw, Poland;5. Department of Pediatrics Oncology, Children’s Memorial Health Institute, Warsaw, Poland, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland;1. Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT, USA;2. Department of Pharmacy, Yale New Haven Health, New Haven, CT, USA;3. Department of Internal Medicine, Section of Hematology, Yale School of Medicine, New Haven, CT, USA;4. Department of Pediatrics, Yale School of Medicine, New Haven, CT, USA;1. Hematology Department, Faculty of Medicine, King Abdulaziz University, P.O. Box 80216, Jeddah, 21589, Saudi Arabia;2. Blood Transfusion Services, King Abdulaziz University Hospital, King Abdulaziz University, P.O. Box 80216, Jeddah, 21589, Saudi Arabia;3. Neurooncology Translational Group, King Fahd Medical Research Center, Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80216, Jeddah, 21589, Saudi Arabia;4. Department of Hematology, Sultan Qaboos University Hospital, Muscat, Oman;5. Blood Bank, Alfateh Children Hospital, Benghazi, Libya;6. Medical Affairs, Sheikh Khalifa Medical City, 51900, Abu Dhabi, United Arab Emirates |
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| Abstract: | BackgroundPlatelets (PLTs) stored at 20–24 °C have a short shelf life of only 5 days, which can result in their restricted availability. PLT cryopreservation extends the shelf life to 2 years.MethodsWe implemented a method of PLT freezing at ?80 °C in 5–6% dimethyl sulfoxide. Buffy-coat-derived leucodepleted fresh PLTs blood group O (FP) were used for cryopreservation. Cryopreserved pooled leucodepleted PLTs (CPP) were thawed at 37 °C, reconstituted in PLT additive solution SSP + and compared to FP regarding PLT content, PLT concentration, pH, volume, PLT loss, anti-A/B antibody titre, total protein, plasma content, and PLT swirling. Clot properties were evaluated via rotational thromboelastometry. PLT microparticle number and surface receptor phenotype were assessed via flow cytometry.ResultsCPP met the required quality parameters. The mean freeze-thaw PLT loss was 22.24 %. Anti-A/B antibody titre and plasma content were significantly lower in CPP. CPP were characterised by faster clot initiation and form stable PLT clots. The number of PLT microparticles increased 25 times in CPP and there were more particles positive for the activation marker CD62 P compared to FP.ConclusionThawing and reconstitution are easy and fast processes if platelet additive solution is used. Low anti-A/B antibody titre and plasma content make possible the use of CPP of blood group O reconstituted in SSP + as universal ABO products, including clinical situations where washed PLTs are required. Clot properties evaluated via rotational thromboelastometry demonstrated that CPP retain a significant part of their activity compare to FP and are haemostatically effective. |
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| Keywords: | Cryopreserved platelets Buffy-coat-derived platelets Platelet freezing Platelet microparticles |
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