食管鳞癌nm23-H1及基质金属蛋白酶-7蛋白表达的临床病理意义

目的探讨nm23-H1及基质金属蛋白酶-7(MMP-7)的表达与食管癌发生、发展及浸润、转移的关系。方法应用免疫组织化学SP法检测60例食管鳞癌组织、30例癌旁不典型增生组织及60例正常食管黏膜组织中nm23-H1及MMP-7蛋白表达。结果食管鳞癌组织中nm23-H1蛋白表达与癌的浸润深度及淋巴结转移密切相关(P<0.05);MMP-7蛋白表达与癌的组织学分级、浸润深度及淋巴结转移密切相关(P<0.05);在食管上皮癌变过程中nm23-H1蛋白表达在正常黏膜组织、癌旁不典型增生组织及癌组织中的表达率依次降低,分别为95.0%(57/60)、56.7%(17/30)、21.7%(13/60),组间比较有明显差异(P<0.05);而MMP-7蛋白在癌组织及癌旁不典型增生组织表达率显著高于正常黏膜组织,分别为73.3%(43/60)、80.0%(24/30)、36.7%(22/60),组间比较有明显差异(P<0.05);nm23-H1与MMP-7的表达呈负相关关系(P<0.05)。结论nm23-H1和MMP-7在食管癌的浸润、转移及黏膜上皮癌变过程中起重要作用,nm23-H1及MMP-7的联合检测有望成为食管鳞癌早期诊断和判断预后的分子指标之一。

Clinical pathological significance of the expression of nm23-H1 and matrix metalloproteinase -7 in esophageal squamous cell carcinoma

Objective To explore the relationship of the expression of nm23-H1 and matrix metalloproteinase-7(MMP-7) protein,the occurrence,development,invasion and metastasis in esophageal squamous cell carcinoma.Methods SP immunohistochemical method was used to detect the expression of nm23-H1 and MMP-7 protein in 60 cases of esophageal squamous cell carcinoma specimens,30 cases of adjacent atypical hyperplasia epithelium and 60 cases of normal esophageal epithelium.Results The expression of nm23-H1 was closely correlated with infiltration depth and lymphatic metastasis in esophageal squamous cell carcinoma(P<0.05).The expression of MMP-7 protein was closely correlated with the tumor histological grade,infilatration depth and lymphatic metastasis in esophageal squamous cell carcinoma(P<0.05).In the esophagus epidermis canceration process,the expression rates of nm23-H1 in normal esophageal epithelium specimens,adjacent atypical hyperplasia epithelium and carcinoma reduced in turn which were 95.0%(57/60)、56.7%(17/30)、21.7%(13/60)respectively.And there was significant difference in group comparison(P<0.05).The positive rate of MMP-9 protein expression in cancer tissue and mucosa adjacent to cancer were higher than that in normal mucosa tissue which were 73.3%(43/60)、80.0%(24/30)、36.7%(22/60) respectively.And there was significant difference in group comparison(P<0.05).There was negative correlation between the expression of nM23-H1 and MMP-7(P<0.05).Conclusion nm23-H1 and MMP-7 play important roles in infiltration,metastasis and mucous membrane epidermis canceration process of esophageal carcinoma.nm23-H1 and MMP-7 can become one of molecular index in the early diagnosis and prognostic judgement of esophageal squamous cell carcinoma.