CpG寡脱氧核苷酶对2型糖尿病患者外周血Th1/Th2型免疫应答的调节作用

目的观察CpG寡脱氧核苷酶(ODN)对2型糖尿病(T2DM)患者外周血单个核细胞(PBMC)γ干扰素(IFN-γ)、白细胞介素(IL)-12以及IL-10表达的影响。方法将20例T2DM患者和20名健康人PBMC根据刺激物不同分为空白对照组、CpG ODN组(2μmol/L)。用实时足量聚合酶链反应(real time-PCR)法检测PBMC IFN-γmRNAI、L-12 mRNA和IL-10 mRNA的表达。结果T2DM患者PBMC空白对照组IFN-γmRNA和IL-12 mRNA的表达低于健康人(P<0.01,P<0.05),IL-10 mRNA的表达与健康人相似(P>0.05)。经CpGODN刺激,IFN-γmRNA和IL-12 mRNA的表达高于空白对照组(P<0.01,P<0.01),但低于健康人(P<0.01,P<0.05),IL-10 mRNA的表达与空白对照组相似(P>0.05),与健康人差异无统计学意义(P>0.05)。结论CpG ODN可促进T2DM人PBMC产生IFN-γ和IL-12,增强Th1型免疫反应。

Modulatory effects of CpG oligonucleotide on production of Th1 and Th2 cytokines by peripheral blood mononuclear cells from patients with type 2 diabetes mellitus

Objective To evaluate the effects of CpG oligonucleotide on the production of interferon γ(IFN-γ),interleukin(IL)-12 and IL-10 in peripheral blood mononuclear cells(PBMC) in patients with type 2 diabetes mellitus(T2DM).Methods PBMC was isolated from 20 patients and 20 healthy donors,and then cultured in RPMI-1640 without stimulator(control) or CpG ODN(2 μmol/L) respectively.The expression of IFN-γ mRNA,IL-10 mRNA and IL-12 mRNA in PBMC were detected by real time-PCR.Results In control group,the expression of IFN-γ mRNA and IL-12mRNAinpatientswaslowerthanthatin healthy donors (P<0.01,P<0.05),and the expression of IL-10 mRNA in patients was similar to that in healthy donors (P>0.05).In CpG ODN group,the expression of IFN-γ mRNA and IL-12 mRNA in patients were higher than that in control groups (P<0.01,P<0.01), the expression of IFN-γ mRNA and IL-12 mRNA in patients was lower than that in healthy donors (P<0.01,P<0.05), and the expression of IL-10 mRNA in patients was similartothatincontrolgroups and healthy donors (P>0.05,P>0.05).Conclusion ①There are decreased ability of production of Th1-type cytokines(IFN-γ and IL-12) and increased potential for Th2-type cytokine(IL-10) in PBMC from patients with T2DM.②CpG ODN could increase the production of IFN-γ and IL-12,and decrease the production of IL-10.