| 胚鼠大脑皮质神经元的最佳分离和培养方法的探讨 |
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| 引用本文： | 李胜富,毛萌,周晖,李幼平,孙明菡. 胚鼠大脑皮质神经元的最佳分离和培养方法的探讨[J]. 生物医学工程学杂志, 2001, 18(3): 422-424,431,F002 |
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| 作者姓名： | 李胜富 毛萌 周晖 李幼平 孙明菡 |
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| 作者单位： | 1. 四川大学华西医院
2. 四川大学华西第二医院儿科 |
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| 基金项目： | CMB基金 (00722)、四川省人事厅学术和技术带头人基金(49899021)资助课题 |
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| 摘 要： | 用不同类型酶消化法及单纯机械法分离胚鼠大脑皮质,并分别采用两种不同的培养基进行神经元的体外培养以建立胚鼠脑皮质神经元的最佳分离和培养方法,结果表明：采用0.05%胰蛋白酶＋0.05%Ⅱ型原酶消化,以神经元完全培养基作为培养基质可获得高纯度、高活性的大脑皮质神经元。
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| 关 键 词： | 胚鼠 皮质神经元 分离 原代培养 |
A Study for Optimal Method of Separating and Cultivating Embryonic Rat Cerebral Neurons |
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| Li Shengfu Mao Meng Zhou Hui Li Youping Sun Minghan. A Study for Optimal Method of Separating and Cultivating Embryonic Rat Cerebral Neurons[J]. Journal of biomedical engineering, 2001, 18(3): 422-424,431,F002 |
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| Authors: | Li Shengfu Mao Meng Zhou Hui Li Youping Sun Minghan |
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| Affiliation: | Laboratory of Transplant Engineering and Immunology, West China Hospital of Sichuan University, Chengdu 610044. |
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| Abstract: | In order to improve the method of separating and cultivating the embyronic rat cerebral neurons in vitro, we compared different digestive enzymes, mechanical separating methods and two different mediums. It was found that highly purified and active embryonic rat cerebral neurons could be harvested by cultivating the neurons in the standard neurobasal medium with B 27 supplement ,10% horse serum, 10% fetal bovine serum and L Glutamine after separated by digestion with the use of 0.05% trypsin and 0.05% collagenase II. |
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| Keywords: | Embryonic rat cerebral neurons Separation Primary culture |
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