全反式维甲酸对人卵巢癌细胞株HO8910增殖、侵袭能力的影响

目的:观察全反式维甲酸(All-trans retinoic acid,ATRA)对体外培养人卵巢癌细胞株HO8910增殖、侵袭能力的影响,为ATRA应用于卵巢癌的临床治疗提供实验依据.方法:选用人卵巢癌细胞株HO8910进行体外培养,以不同浓度的ATRA处理HO8910细胞,四甲基偶氮唑蓝(MTT)比色法检测细胞增殖抑制情况,倒置显微镜进行形态学观察,Transwell小室体外侵袭实验检测细胞侵袭能力.结果:(1)ATRA浓度为10~(-7)～10~(-5)mol/L时,均明显抑制HO8910细胞的增殖(P<0.05),并具有时间-剂量依赖性.24、48、72 h ATRA抑制HO8910细胞增殖的IC_(50)值分别为2.331×10~(-5)、0.998×10~(-6)、0.891×10~(-7)mol/L.(2)倒置显微镜可观察到经10~(-6)mol/LATRA处理的HO8910细胞部分发生形态学的良性分化.(3)体外侵袭实验显示经10~(-6)mol/L ATRA处理的HO8910细胞,穿膜细胞数目明显减少(P<0.05).结论:ATRA能明显抑制人卵巢癌细胞株HO8910的增殖、侵袭能力,有望为ATRA应用于卵巢癌的临床治疗提供新的有效的途径.

Effects of all-trans retinoic acid on the human ovarian cancer HO8910 cell line

Objective:To investigate the effects of all-trans retinoie acid on the human ovarian cancer cell line HO8910.Methods:The human ovarian cancer cell line HO8910 cultured in vitro were treated with various concentrations of all-trans retinoic acid.The cell proliferation inhibition was detected by MTT assay;The morphologic changes of cell differentiation were observed under inverted microscope;The invasion capability was evaluated by transwell chambers.Resets:1.ATRA of 10~(-7)～10~(-5)mol/L inhibited the proliferation of HO8910 cell significantly in a dose-dependent and time-dependent manner(P<0.05)and its IC_(50) were 2.331 × 10~(-5)mol/L、0.998×10~(-6) mol/L、0.891×10~(-7)mol/L at 24 hours、48 hours and 72 hours,separately.2.Treated by 10~(-6) mol/L ATRA,HO8910 cells showed striking morphological characteristics of differentiation under microscope.3.The number of invasive cells decreased significantly after treated by 10~(-6) mol/L ATRA(P<0.05).Conclusion:ATRA can both inhibit the proliferation and invasion of human ovarian cancer cell line HO8910.Which expect to provide an effective and novel approach for clinical treatment of human ovarian cancer.