MCP-1、ICAM-1在糖尿病肾病大鼠肾脏损害中作用及厄贝沙坦干预的影响

目的：探讨MCP-1、ICAM-1在糖尿病肾病大鼠肾脏损害中作用及厄贝沙坦对二者的影响.方法：采用高糖高脂饮食合并链脲佐菌素腹腔注射的方法建立糖尿病肾病大鼠模型.将大鼠随机分为正常对照NC 组、糖尿病肾病DN组、厄贝沙坦DI组,检测各组大鼠24 h尿量、24 h尿白蛋白定量（24 h UTP）、血糖（BG）、血肌酐（Scr）、尿素氮（BUN）、肾重指数（KI）;行HE染色观察各组大鼠病理学形态,免疫组化观察MCP-1、ICAM-1蛋白的表达,RT-PCR观察MCP-1 mRNA、ICAM-1mRNA的表达.结果：与NC组比较,DN组大鼠肾脏病理改变加重,24 h尿量、24 h UTP、KI、BG、Scr、BUN、肾脏组织中MCP-1mRNA和ICAM-1mRNA及蛋白水平均显著增加,差异均有统计学意义（P＆lt;0.01）;与DN组比较,DI组大鼠BG、BUN、Scr有所改善,差异无统计学意义;肾脏病理改变减轻,其余指标明显降低,差异有统计学意义（P＆lt;0.05）.结论：MCP-1、ICAM-1在糖尿病肾病肾脏损害过程中可能起重要作用;厄贝沙坦能够减轻糖尿病肾病肾组织MCP-1、ICAM-1的表达,缓解了肾脏病理损伤.

The Role of MCP-1and ICAM-1 in Kidney Damaged Diabetic Nephropathy Rats and the Intervention Effect of Irbesartan

Objective： This experiment studied the role of monocyte chemoattractant protein - 1 （ MCP - 1 ） and intercellular adhesion molecule - 1 （ ICAM - 1 ） in Kidney damaged diabetic nephropathy rats and the effect of irbesartan on them. Methods： Using the high - sugar and high - fat diets combined with intraperitoneal injection of Streptozotocin to establish diabetic nephropathy rats model. Rats were randomly divided into normal control NC group, diabetic nephropathy DN group, irbesartan DI group ,Testing 24 h urine volume, 24 h urine protein quantitative （24 h UTP） , blood glucose （BG）, blood muscle anhydride （Ser）, urea nitrogen （ BUN）, and index of kidney （ renal/weight, KI） ; Using HE stain to observe pathological morphology of each group, immunohisto- chemistry to observe the protein expression of MCP - 1 and ICAM - 1, RT - PCR to observe their mRNA expression. Results： Com- pared with the NC group, kidney pathological changes of DN group aggravated, 24 h urine volume, 24 h UTP, BG, Scr, BUN, KI and expressions of MCP - 1 and ICAM - 1 mRNA and protein in the renal tissues were significantly increased , with statistically significant differences（ P 〈 0.01 ） ; Compared with the DN group, BG, Scr and BUN of DI group relatively improved , with no statistically sig- nificant differences. Kidney pathological changes alleviated. The rest indices evidently reduced, with statistically significant differ- ences （ P 〈 0.05 ）. Conclusion： MCP - land ICAM - 1 may play an important role in the process of kidney damage in diabetic nephropathy ; Irbesartan can reduce expressions of MCP - 1 and ICAM - 1 in diabetic renal tissues, to some extent relieving the pathological damage of the kidney.