人参果皂甙对失血性休克犬心肌的保护作用

用杂种犬16只分为失血性休克组(HS)和失血性休克人参果皂甙组(HSG)。HS组动物由一侧颈总动脉放血至血压40mmHg(5.33kPa),放血后4h内采用放血和输血的方法维持血压。HSG组动物于放血前30min静脉注射人参果皂甙,HS组动物静脉注射等量的生理盐水,其它实验过程同HS组。两组动物存活时间,处死前血压和终失血量均存在显著性差异(P<0.005,P<0.05,P<0.001)。动物死亡后观察心肌细胞内某些酶的含量变化,HSG组动物心肌磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)和细胞色素氧化酶(CCO)的含量明显高于HS组。电镜观察:HS组动物心肌细胞的肌膜、核膜和线粒体均有不同程度的损伤,而HSG组动物心肌细胞的肌膜、核膜和线粒体基本正常。以上结果表明:人参果皂甙具有抗休克和保护心肌的作用。

Protective effect of ginseng fruit saponin on myocardium in hemorrhagic shock dogs

Sixteen hybrid dogs were randomly divided inio two groups: (1) HSG grouphemorrhagic shock pretreated with ginseng fruit saponin at 30 min before bleeding and(2) HS group, hemorrhagic shock pretreated with same volum of saline. The hemorrhagicshock was induced by bleeding from carotid artery. Mean arterial blood pressure of 5. 3 kPawas maintained for 4 hr. In there 2 group of animals, the survival rates average pressurebefore put to death, end hemorrhage were obviously all different (P<0. 005. P<0. 05. P<0. 001). The enzymic changes of myocardial cell were observed, after animial death. Theactivities of CK (phosphocreatine kinase), LDH (lactic dehydrogenase), SDH (succinate dehydro-genase) and CCO (cytochrone oxidase) of SHG group were obviously higher than that ofthe SH group. Observation of myocardium under electron microscope showed that sarcolemma,nuclear membrane and mitochondria in SH group were all damaged, but no obvious changeobserved in the SHG group. These results suggested that ginseng fruit saponin had resistedshock and had protective effort on myocardium.