Protoplast fusion in a petite-negative yeast,Kluyveromyces lactis |
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Authors: | Andrew J Morgan Aurora Brunner Peter A Whittaker |
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Institution: | (1) The School of Biological Sciences, University of Sussex, BN1 9QG Falmer, Brighton, England;(2) Depto. de Microbiologia, Centro de Investigaciones en Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Apdo. Postal 70-600, Mexico 20, D. F.;(3) Department of Biology, St. Patrick's College, Maynooth, Co. Kildare, Ireland |
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Abstract: | Summary The technique of protoplast fusion has been applied to the problem of unstable diploidy in the yeast Kluyveromyces lactis. By protoplast fusion between heterothallic strains of like mating-type, sporulation-deficient hybrids can be obtained. Biochemical, cytological, and genetical characterisation of these hybrids suggests that the majority of fusion products are diploid. Sporulating hybrids can be constructed by protoplast fusion between homothallic strains. Tetrad analysis of these hybrids demonstrates conclusively the diploid nature of fusion products. |
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Keywords: | Protoplast fusion Kluyveromyces Hybrid characterization Stable Diploids |
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