用AB/PAs、HID、CEA法提高胃粘液细胞癌淋巴结转移检出率的研究

用AB(pH2.5)/PAS、HID及CEA(ABC法)对48例胃粘液细胞癌及其淋巴结进行染色观察,对照HE染色,结果表明应用上述方法染色可提高淋巴结转移检出率33.1%,其中AB(pH2.5)/PAS法显示了很强的特异性和敏感性,较HID和CEA法优越。淋巴结微小转移灶多为PAS阳性细胞,说明幼稚的癌细胞分泌中性粘液、侵袭力强、容易转移并先发生转移。CEA标记结果显示本肿瘤有分化越差、越幼稚其CEA含量越少的现象。

Increases the Positive Rates of Metastases Detection lymph Nodes of Gastric Mucocellular Carcinoma by Means of AB/PAS, HID and CEA

Using AB(pH2. 5)/PAS, HID and CEA(ABC) staining for 48 cases of gastric mucocellular carcinomas and their lymph nodes, compared with HE staining. The results showed that the positive rate of lymph nodes metastases was increased by 33.1%. The AB(pH2.5)/PAS staining was more specific and sensitive than HID and CEA. In metastatic lymph nodes, the tumor cells were of major PAS positive. It demonstrated that the invasion ability of infantile tumor cell is strong and their metastases easily and usually formed at early stage. The result of CEA staining suggests that the lower degree of the tumor cell differentiation is the less the amount of CEA.