脂肪间充质干细胞的成骨诱导分化

目的　研究脂肪间充质干细胞 (MSCs)在特定培养条件下向成骨细胞分化 ,探讨其作为骨组织工程的种子细胞的可行性。方法　取 3周龄Lewis大鼠的腹股沟脂肪垫 ,消化法获得脂肪MSCs,用成骨诱导培养基诱导其向成骨细胞分化 ,组织化学染色、免疫细胞化学染色和Westernblotting检测细胞分化的情况。结果　从成体大鼠脂肪组织中培养出脂肪MSCs,能大量稳定增殖传代。在地塞米松、抗坏血酸、β-甘油磷酸钠的诱导下 ,脂肪MSCs的ALP活性增高 ,VonKossa染色出现钙结节 ,OPN、BMP - 2免疫细胞化学染色阳性 ,Westernblotting检测到诱导后细胞OPN、BMP - 2的表达 ,且随诱导时间延长表达增强。结论　从脂肪组织中可获得具有多分化潜能的MSCs,并能在体外稳定增殖传代 ,经诱导后可分化为脂肪细胞和成骨细胞 ,有可能成为骨组织工程较理想的种子细胞之一

The Osteoblast Differentiation of Adipose Mesenchymal Stem Cells

Objective To investigate osteoblast differentiation of adipose mesenchymal stem cells as seeding cells in bone tissue engineering.Methods Adipose mesenchymal stem cells were obtained from inguinal fat pads of Lewis rat with digestion.Adipose mesenchymal stem cells were induced into osteoblasts by revulsant,and then the differentiations were examined with cytochemistry,immuncytochemistry and Western blotting.Results A population of adipose mesenchymal stem cells were successfully isolated from rat adipose tissue.They appeared fibroblast-likely and could stably proliferated and passaged in vitro.After treated with dexamethasone,ascorbic acid,and beta-sodium glycerophosphate,the differentiation of adipose mesenchymal stem cells towards osteoblasts was verified by the positive results of ALP,OPN and BMP-2 through cytochemistry,immuncytochemistry and Western blotting respectively.Conclusion Mesenchymal stem cells with multilineage potential obtained from rat adipose tissue can differentiate to osteoblasts when cultured in vitro,and adipose mesenchymal stem cells can likely be served as optimal autogenous cell source for bone tissue engineering.