| HPLC法测定小承气汤中大黄酸和厚朴酚的含量 |
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| 引用本文： | 胡晓静,高轶哲,李飞,赵春杰. HPLC法测定小承气汤中大黄酸和厚朴酚的含量[J]. 沈阳药科大学学报, 2007, 24(1): 26-28 |
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| 作者姓名： | 胡晓静 高轶哲 李飞 赵春杰 |
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| 作者单位： | 1. 沈阳药科大学,药学院,辽宁,沈阳,110016
2. 深圳市制药厂,广东,深圳,518029 |
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| 摘 要： | 目的建立小承气汤中大黄酸和厚朴酚的HPLC含量测定方法。方法样品以体积分数为70%的乙醇回流提取,采用Thermo C18柱(250 mm×4.6 mm,5μm),流动相为甲醇体积分数为1%乙酸水溶液(体积比为72∶28),流速为1.0 mL.min-1,检测波长为254 nm。结果大黄酸和厚朴酚的线性范围分别为1.52~19.0 mg.L-1和1.68~21.0 mg.L-1,平均回收率为97.6%和101.3%。结论建立的方法可用于小承气汤的质量控制。
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| 关 键 词： | 高效液相色谱法 小承气汤 大黄酸 厚朴酚 |
| 文章编号： | 1006-2858(2007)01-0026-03 |
| 修稿时间： | 2006-07-07 |
Determination of the contents of rhein and magnolol in the Xiaochengqi decoction by HPLC |
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| HU Xiao-jing,GAO Yi-zhe,LI fei,ZHAO Chun-jie. Determination of the contents of rhein and magnolol in the Xiaochengqi decoction by HPLC[J]. Journal of Shenyang Pharmaceutical University, 2007, 24(1): 26-28 |
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| Authors: | HU Xiao-jing GAO Yi-zhe LI fei ZHAO Chun-jie |
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| Affiliation: | 1, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China 2. Shenzhen Pharmaceutical factory, Shenzhen 518029, China |
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| Abstract: | Objective To establish a reversed-phase HPLC method for determination of rhein and magnolol in Xiaochengqi decoction.(tradi tional Chinese medi cines)Methods The sample was separated on a Thermo-C_(18) column(250 mm×4.6 mm,(5 μm))using methanol-1% acetic acid solution(V∶V=72∶28) as the mobile phase at a flow rate of 1.0 mL·min~(-1).The detection was set at 254 nm.Results The calibration curve was linear in the range of 1.5219.0 mg·L~(-1)(r=0.999 7) for the rhein and 1.68-21.0 mg·L~(-1) for the magnolol(r=0.999 6).The inner-day RSD was 0.15% and 0.14%,and the average was 97.6% and 101.3% respectively.Conclusions This method is accurate,simple and sensitive,and can be used for the quality control of Xiaochengqi decoction. |
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| Keywords: | HPLC Xiaochengqi decoction rhein magnolol |
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