儿童血管瘤内皮细胞功能测定及病因学意义

目的　探讨血管瘤内皮细胞功能及其病因学意义。方法　采用放射免疫和硝酸还原酶法测定儿童血管瘤 30例瘤体内皮素 (ET)、一氧化氮 (NO)及外周血ET、NO水平 ,以 2 0例斜疝患儿外周血作为对照。结果　血管瘤患儿瘤体ET(6 6 .3± 12 .5 )pg/ml显著高于外周血 (5 5 .3± 13.1)pg/ml(P <0 .0 1) ,外周血ET与对照组 (5 4 .4± 15 .1) pg/ml相比差异无显著性意义 (P >0 .0 5 )。增生期血管瘤体ET(75 .6± 12 .5 ) pg/ml高于退化期 (6 5±10 .2 ) pg/ml及退化完成期 (6 2 .5± 7.3) pg/ml(P <0 .0 5 )。血管瘤体NO(32 .5± 9.3) pg/ml与外周血 (37.5± 5 .2 ) pg/ml和正常对照组 (39.5±8.8) pg/ml无差异 (P >0 .0 5 ) ,但增生期瘤体NO(2 7.9± 2 .1) pg/ml降低 (P <0 .0 1)。结论　增生期血管瘤内皮细胞具有活跃的合成分泌ET的功能 ,而合成分泌NO功能改变不明显 ,局部ET与NO水平的失调 ,可能与血管内皮的过度增生有关

Function of endothelial cell and pathogenesis of hemangioma in children

Objective To investigate the status of vascular endothelial cell in proliferative and involutive hemangioma.Methods Thirty cases of hemangioma were compared with a control group of 20 children with inguinal hernia. Endothelins (ET) and Nitric Oxide (NO) in the hemangioma were measured with radioimmunoassay and nitrate reductase respectively. ET and NO of blood plasma in all subjects were detected by the similarly.Results The level of ET in proliferative hemangioma was higher ( P < 0.01 ) than that of involutive hemangioma while the level of NO in the proliferative hemangioma was lower ( P < 0.05 ).Conclusions ET is actively secreted by endothelial cells of hemangioma during the proliferative phase. No obvious change of NO is detected. The imbalance of the ET and NO may be the cause of the endothelial cell's excessive proliferation.