| 应用人—鼠杂交瘤技术制备抗绿脓杆菌人单克隆抗体的初步研究 |
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| 引用本文： | 叶丹霓 彭海荣. 应用人—鼠杂交瘤技术制备抗绿脓杆菌人单克隆抗体的初步研究[J]. 华西医科大学学报, 1991, 22(1): 24-26 |
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| 作者姓名： | 叶丹霓 彭海荣 |
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| 摘 要： |
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| 关 键 词： | 杂交瘤技术 绿脓杆菌 单克隆抗体 |
The production of human monoclonal antibodies against Pseudomonas aeruginosa by human-mouse hybridoma technique] |
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| D Ye,R Yan,S Li,H Peng,X Xu. The production of human monoclonal antibodies against Pseudomonas aeruginosa by human-mouse hybridoma technique][J]. Journal of West China University of Medical Sciences, 1991, 22(1): 24-26 |
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| Authors: | D Ye R Yan S Li H Peng X Xu |
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| Affiliation: | Research Unit of Leptospirosis. |
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| Abstract: | The fusion of peripheral B lymphocytes from human immunized with P. aeruginosa polyvalent vaccine and mouse myeloma cell line SP2/0 was successfully performed. The rate of fusion was 74%(71/96) and the positive rate of antibody was 19.7%. Two hybridoma cell lines (A3 and F8) secreting McAb against P. aeruginosa were obtained after three times cloning by limiting dilution. The human chromosomes together with mouse chromosomes were discovered in karyotype assay of the hybrids. A3 and F8McAbs were human IgG by class determination. These MrcAbs could recognize 43 kd and 36 kd MW specific components of P. aeruginosa antigen by enzyme linked immuno-transfer blot technique. |
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