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反相液相色谱法测定中成药中盐酸小檗碱
引用本文:郭平,李章万.反相液相色谱法测定中成药中盐酸小檗碱[J].华西医科大学学报,1991,22(1):90-92.
作者姓名:郭平  李章万
摘    要:

关 键 词:盐酸小檗硷  黄连  中成药  色谱法

Determination of berberine hydrochloride in traditional Chinese medicines containing Coptis chinensis Franch by reversed phase high performance liquid chromatography]
P Guo,Z Li,Z Hong,S Liu,T Wu.Determination of berberine hydrochloride in traditional Chinese medicines containing Coptis chinensis Franch by reversed phase high performance liquid chromatography][J].Journal of West China University of Medical Sciences,1991,22(1):90-92.
Authors:P Guo  Z Li  Z Hong  S Liu  T Wu
Abstract:This paper reports a reversed phase high performance liquid chromatography method to determine berberine hydrochloride in Coptis chinensis Franch and in the traditional Chinese medicine containing Coptis chinensis Franch. The result shows that the mobile phase of 0.4mol/L ammonium nitratemethanol (52:48) can give a satisfactory separation. The extraction of sample with 1% H2SO4 solution using the ultrasonic vibration method is simple and easy to operate.
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