高功率微波辐射后下丘脑神经元凋亡和线粒体膜电位与Ca2+的变化

目的探讨高功率微波辐射后下丘脑神经元凋亡、线粒体膜电位与Ca^2＋的变化。方法以10与30mW／cm^2高功率微波辐射原代培养的下丘脑神经元，于辐射后6h采用倒置显微镜和流式细胞术检测细胞的凋亡、线粒体膜电位与胞浆钙离子的变化。结果辐射后6h，10与30mW／cm^2组凋亡率与假辐射组相比均明显增加；30mW／cm^2组辐射后坏死率与假辐射组比较亦明显升高，差异有统计学意义（P〈0．05）。辐射后6h，30mW／cm^2组Ca^2＋明显升高，膜电位性明显下降，差异均有统计学意义（P〈0．01）。结论细胞凋亡是下丘脑神经元死亡的主要方式之一，胞浆内Ca^2＋超载及线粒体膜电位的下降参与其损伤过程。

Changes of apoptosis, mitochondrion membrane potential and Ca2+ of hypothalamic neurons induced by high power microwave

OBJECTIVE: To explore the injury effect and mechanism of hypothalamic neurons after high power microwave (HPM) exposure. METHODS: Primarily cultured hypothalamic neurons were exposed to 10 and 30 mW/cm(2) HPM, and the inverted phase contrast microscope (IPCM) and flow cytometry (FCM) were employed to detect the injury of cells and change of mitochondrion membrane potential (MMP) and Ca(2+) in the cytoplasm of neurons. RESULTS: The ratio of apoptosis was significantly higher than that of the sham exposure (P < 0.05) induced by 10 and 30 mW/cm(2) HPM and necrosis increased significantly (P < 0.05) in the group of 30 mW/cm(2) at 6 h after exposure. The content of Ca(2+) in the cytoplasm of neuron cells increased (P < 0.01) while MMP decreased significantly (P < 0.01) after radiation of 30 mW/cm(2) HPM at 6 h after exposure. CONCLUSION: Apoptosis is one of the major death ways of hypothalamic neurons. The overloading of Ca(2+) and the decline of MMP are involved in the process.