Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase
of Heat Shock Protein 70 Expression in Rat Articular Cartilage |
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| Authors: | Ki Won Nam Dong Yel Seo Min Hee Kim |
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| Affiliation: | 1) Department of Physical Therapy, College of Health and Welfare, Dongshin University, Republic of Korea;2) Department of Physical Therapy, Graduate School of Dongshin University, Republic of Korea;3) Department of Physical Therapy, College of Health Science, Eulji University, Republic of Korea |
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| Abstract: | [Purpose] The present study was aimed to investigate the effects of pulsed andcontinuous ultrasound (US) irradiation on heat shock protein (HSP) 70 and mRNA levels ofchondrogenesis-related gene expression in rat tibial articular cartilage. [Subjects andMethods] Forty-eight rats with body weights of 200−250 g were randomly divided into threegroups. In the control (CON) group, three rats were treated with sham sonication. Thepulsed US irradiation group was irradiated with a pulse rate of 20%, a frequency of 1 MHz,and an intensity of 1.5 W/cm2 for 10 minutes. The continuous US irradiationgroup was continuously with a frequency of 1 MHz and an intensity of 1.5 W/cm2for 10 minutes. Immunohistochemistry for evaluation of HSP 70 and RT-PCR for expression ofthe chondrogenesis-related mRNA were used. [Results] The expression of HSP70 protein wasincreased in the pulsed and continuous US groups. The increase in the continuous US groupwas more prominent than in the pulsed US group. In addition, pulsed and continuous USirradiation increased the expression of Mustn1 and Sox9. [Conclusion] The results of thisstudy show that US increases chondrogenesis via the increase of HSP 70 andchondrogenesis-related mRNA expressions in rat articular cartilage.Key words: Articular cartilage, Heat shock protein 70, Ultrasound |
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