From the Cover: Membrane raft association is a determinant of plasma membrane localization |
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Authors: | Blanca B Diaz-Rohrer Kandice R Levental Kai Simons Ilya Levental |
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Institution: | aDepartment of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, TX, 77030;;bMax Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany; and;cCancer Prevention and Research Institute of Texas, Austin, TX, 78701 |
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Abstract: | The lipid raft hypothesis proposes lateral domains driven by preferential interactions between sterols, sphingolipids, and specific proteins as a central mechanism for the regulation of membrane structure and function; however, experimental limitations in defining raft composition and properties have prevented unequivocal demonstration of their functional relevance. Here, we establish a quantitative, functional relationship between raft association and subcellular protein sorting. By systematic mutation of the transmembrane and juxtamembrane domains of a model transmembrane protein, linker for activation of T-cells (LAT), we generated a panel of variants possessing a range of raft affinities. These mutations revealed palmitoylation, transmembrane domain length, and transmembrane sequence to be critical determinants of membrane raft association. Moreover, plasma membrane (PM) localization was strictly dependent on raft partitioning across the entire panel of unrelated mutants, suggesting that raft association is necessary and sufficient for PM sorting of LAT. Abrogation of raft partitioning led to mistargeting to late endosomes/lysosomes because of a failure to recycle from early endosomes. These findings identify structural determinants of raft association and validate lipid-driven domain formation as a mechanism for endosomal protein sorting.Recent advances in superresolution microscopy (1), lipid analysis (2, 3), and plasma membrane (PM) isolation (4, 5) have confirmed the coexistence of lipid-driven, fluid domains in biological membranes. The relatively ordered domains, known as “membrane rafts,” have been proposed to be involved in protein sorting (6), viral/pathogen trafficking (3, 7), and PM signaling in a variety of contexts (8). However, despite the increasing evidence confirming the existence of dynamic, nanoscopic membrane rafts, the functional consequences of this phenomenon remain speculative because of the limitations of the previously used methods for defining raft association, i.e., the resistance of membrane components to solubilization by nonionic detergents (9).Lipid-mediated domains have been implicated as a mechanism for protein sorting in the latter stages of the secretory pathway (trans-Golgi network to the PM) (2, 6, 10–12), with analogous pathways mediating endosomal sorting/recycling (13, 14). Raft lipids (i.e., sterols and sphingolipids) are significantly enriched at the PM (15–17), and recent observations confirm that these lipids also are enriched in sorting vesicles destined for the PM (2, 11). For proteins, several specific cytosolic signals exist for adapter/coat-mediated sorting between cellular organelles (18); in parallel, protein–lipid interactions through hydrophobic transmembrane domains (TMDs) also have been shown to regulate trafficking. For example, a strong correlation exists between the TMD length of bitopic proteins and their organelle specificity (19, 20), with longer TMDs targeting proteins to the PM and shorter TMDs found in the endoplasmic reticulum (ER), Golgi apparatus, and endocytic organelles. These findings suggest cargo sorting in the secretory and endocytic pathways, with proteins containing longer TMDs, together with sphingolipids and cholesterol, being specifically trafficked to the PM, although the mechanism for this observation remains unresolved.One possibility for sorting of specific lipid classes along with proteins containing longer TMDs is lateral segregation and coalescence of ordered domains, followed by either domain-induced (21) or cytoskeleton-assisted (22) budding of raft-enriched transport vesicles. Proteins using this “raft pathway” would not require cytosolic sorting signals but rather would be recruited to transport vesicles by their raft affinity, i.e., their propensity to interact with specific lipids, ordered domains, or other raft-embedded proteins. Because ordered phases in lipid model systems consistently have been shown to be 0.6–1.5 nm thicker than disordered domains (23, 24), raft-associated transmembrane (TM) proteins would be predicted to have longer TMDs. TMD length-dependent protein sorting between coexisting lipid domains has been addressed experimentally only recently by measuring partitioning of an oligomeric toxin (perfringolysin O) with multiple (35–40) TM segments in synthetic, phase-separated liposomes (25). Whether these observations extend to single-pass TM proteins in biological membranes is unknown.To evaluate the role of lipid-driven raft domains as a mechanism for subcellular protein sorting, we quantitatively compared the raft association of 30 TM protein variants with their subcellular localization. To quantify raft partitioning of the constructs comprising single-pass TM proteins with varying TMD lengths and sequences, we used giant PM vesicles (GPMVs). GPMVs are cell-detached PM blebs whose protein (26) and lipid (27) diversity mirrors that of the native PM. These PM vesicles separate into coexisting liquid phases (4) with different order (28), which recruit membrane components in accordance with their predicted raft affinity, i.e., saturated lipids, glycosphingolipids (29), glycosylphosphatidyl inositol-anchored proteins (4), and palmitoylated proteins (30) partition to the ordered phase, denoted here as the “raft phase.” Most importantly, these vesicles provide a platform for repeatable, direct, and quantitative analysis of raft partitioning (30), allowing investigation of the structural determinants of raft association and its effect on protein function. We find that perturbation of raft partitioning by three independent means (decreasing TMD length, mutation of palmitoylation sites, and TMD sequence manipulation) perturbed subcellular localization, leading to missorting of PM proteins to late endosomes and lysosomes because of a failure to recycle nonraft proteins from early endosomes (EEs). These results confirm the presence of a raft-mediated recycling route in nonpolarized cells, begin to define the molecular parameters for protein association with raft domains, and suggest an explanation for the accumulation of proteins with longer TMDs at the PM. |
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Keywords: | membrane domain endocytosis trafficking phase separation microdomains |
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