UHPLC-MS/MS同时检测IBS-D模型大鼠血浆中痛泻要方6种成分的含量及其药动学

目的：建立超高效液相色谱-质谱法（UHPLC-MS/MS）同时测定腹泻型肠易激综合征（IBS-D）模型大鼠血浆中6种成分（芍药苷、橙皮苷、新橙皮苷、升麻素苷、5-O-甲基维斯阿米醇苷、升麻素）的分析方法，并研究这6种成分的药动学特征。方法：采用Phenomenex-C₁₈色谱柱（4.6 mm×50 mm，5 μm），乙腈-0.1%甲酸水溶液为流动相梯度洗脱，流速0.7 mL·min^-1，ESI离子源，柱温35 ℃，SRM检测模式。结果：各成分的线性关系良好（r>0.9974）；精密度RSD<13.89%；血浆中无内源性基质干扰，稳定性良好。6种成分的达峰时间（t_max）分别为（0.5±0），（0.36±0.06），（0.33±0），（0.44±0.08），（0.31±0.13），（0.67±0.26） h，半衰期（t_1/2）分别为（6.63±1.60），（4.31±1.14），（5.43±0.88），（5.98±1.91），（7.80±4.39），（4.33±0.40） h，药峰浓度（C_max）分别为（145.42±9.81），（166.11±4.83），（167.69±2.98），（46.29±3.88），（18.98±1.85），（374.71±7.54） ng·mL^-1。结论：该方法简便、灵敏、准确，分析速度快，可用于痛泻要方的药代动力学研究。

Content determination and pharmacokinetics of six components of Tong-Xie-Yao-Fang in IBS-D model rat plasma by UHPLC-MS/MS

OBJECTIVE To establish an ultra performance liquid chromatography-mass spectrometry(UHPLC-MS/MS)method for the simultaneous determination of six components(paeoniflorin,hesperidin,neohesperidin,cimicifugin,5-O-methylvisamidinoside,cimicifugin)in the plasma of rats with diarrhea-predominant irritable bowel syndrome(IBS-D)and to study the pharmacokinetic characteristics of these six components.METHODS A Phenomenex-C18 column(4.6 mm×50 mm,5μm)was used with a gradient elution of acetonitrile-0.1%formic acid in water as the mobile phase at a flow rate of 0.7 mL·min^-1(ESI ion source,column temperature 35℃,SRM detection mode).RESULTS The linear relationship of each component was good(r>0.9974);RSDs of precision was within 13.89%;there was no endogenous matrix interference in plasma,and the stability was good.The time to peak(tmax)of six components were(0.5±0),(0.36±0.06),(0.33±0),(0.44±0.08),(0.31±0.13),(0.67±0.26)h,respectively;the half-life(t1/2)of them was(6.63±1.60),(4.31±1.14),(5.43±0.88),(5.98±1.91),(7.80±4.39),(4.33±0.40)h,respectively;the peak concentration(Cmax)of them was(145.42±9.81),(166.11±4.83),(167.69±2.98),(46.29±3.88),(18.98±1.85),(374.71±7.54)ng·mL^-1 respectively.CONCLUSION The method is simple,sensitive,accurate and rapid,and can be used for the pharmacokinetic study of Tong-Xie-Yao-Fang.