轻度认知障碍患者淋巴细胞蛋白磷酸酯酶-2A的变化

【目的】探讨轻度认知功能障碍（mildcognitiveimpairment，MCI）患者淋巴细胞蛋白磷酸酯酶-2A（protein洳photase-2A，PP-2A）的变化。【方法】用”P放射性标记法检测了PP-2A的活性，并用免疫印迹法检测不同病程MCI患者及正常对照组血淋巴细胞PP-2A的表达。【结果】1年MCI患者淋巴细胞PP-2A的活性（0．91±0．06）与对照组（1．02-4-0．08）比较有降低（P〈0．05），2年MCI（0．83±0．07）和3年MCI（O．85±O．07）患者淋巴细胞PP-2A的活性与对照组比较有明显降低（P〈O．01，P〈0．01），与1年MCI的比较虽有降低的趋势，但无统计学意义（P〉0．05）。1年MCI组淋巴细胞PP-2A的表达（0．95±0．06）与对照组（1．01±0．07）比较无明显降低（P〉0．05），而2年MCI组（0．85±0．07）及3年MCI组（0．86±0．06）的PP-2A表达与对照组相比明显降低（P〈0．01，P〈0．01），1年MCI组与2年MCI组的PP-2A的表达无明显差别（P〉0．05），与3年MCI组也无差别（P〉0．05）。【结论】MCI患者淋巴细胞PP-2A的活性及表达降低，对MCI的诊断可能有参考价值。

Changes of protein phosphotase-2A in lymphocytes of mild cognitive impairment patient

Objective] To explore the alterations of PP- 2 A in lymphocytes in patients with mild cognitive impairment. Methods] The activity of PP- 2 A was determined by 32 p-labelled in control group, 1 - year MCI group, 2 - year MCI group and 3 - year MCI group, and the expression of PP - 2 A was measured by Westernblot in these groups too. Results] The activity of PP - 2 A was decreased in 1 - year MCI group （0.91 ± 0.06） as compared with controls （ 1.02 ± 0.08） （ P 〈 0.05）. A lower decrease of PP- 2 A activity was also induced in 2 - year MCI group （0.83 ± 0.07） and 3 - year MCI group （0.85 ± 0.07） as compared with controls （P〈0.01, P〈0.01）o There was no obvious difference among the three MCI groups （P 〉 0.05）. In other hand, the expression of PP-2 A was not slgnificandy lower in 1- year MCI group （0.95 ±0.06） as compared with control group （1.01 ± 0.07）, but the expression of PP - 2 A in 2 - year MCI group （0.85 ± 0.07） and 3 - year MCI group （0.86 ±0.06） was lower as compared with control group （ P 〈 0.01, P 〈 0.01）. There was no obvious difference between 1 - year MCI group and 2 - year MCI group （ P 〉 0.05）, and no obvious difference was induced between 1 - year MCI group and 3 - year MCI group （ P 〉 0.05）. Conclusions] Lower expression and activity of PP- 2 A in lymphocytes implies a possible value of MCI diagnosis.