| 聚合酶链原位扩增法检测肺石蜡切片结核菌DNA |
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| 引用本文： | 胡敏,王文亮.聚合酶链原位扩增法检测肺石蜡切片结核菌DNA[J].中华结核和呼吸杂志,1994,17(2):78-79,T009. |
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| 作者姓名： | 胡敏 王文亮 |
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| 作者单位： | 第四军医大学病理学教研室，第四军医大学微生物学教研室 |
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| 摘 要： | 应用并改进聚合酶链反应检测肺石蜡片组织中的微量结核菌，既提高了灵敏性和特异性，又可定位观察病灶及其与周围组织的关系，并可避免放射性污染。适用于尸检，活检（支气管镜检）和涂片标本。
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| 关 键 词： | 结核 基因诊断 聚合酶链反应 |
In situ PCR-gene hybridization method for de-tecting mycobacteium tuberculosis in tissue specimens. |
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| Hu Min,Wang .Wen-linag,Zhao Yi-ling et al..In situ PCR-gene hybridization method for de-tecting mycobacteium tuberculosis in tissue specimens.[J].Chinese Journal of Tuberculosis and Respiratory Diseases,1994,17(2):78-79,T009. |
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| Authors: | Hu Min Wang Wen-linag Zhao Yi-ling |
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| Abstract: | he authors combined the technique ofpolymerase chain reaction(PCR)with hybridizationin situ, and find the method is better,quicker andmore sensitive for deteeting a traee of bacterial DNA,especially for observing the relation between the lesionand the peripheral tissue,the method is more simpleand no radioactive pollution. It seems to be suitablefor detection on samples from autopsy,biopsy andsmears.We suppose themcthod might have a prospectfor clinical diagnosis. |
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| Keywords: | Tuberculosis Immunoenzymetechnics Genes Diagnosis |
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