首页 | 本学科首页   官方微博 | 高级检索  
     

高磷对大鼠甲状旁腺组织及原代培养细胞的刺激作用
引用本文:杨冰,王梅. 高磷对大鼠甲状旁腺组织及原代培养细胞的刺激作用[J]. 中国血液净化, 2008, 7(6): 317-320
作者姓名:杨冰  王梅
作者单位:北京大学人民医院肾内科,北京,100044
摘    要:目的观察高磷刺激下,离体的大鼠甲状旁腺(PTG)组织和培养的原代甲状旁腺细胞的PTH分泌变化规律。方法解剖显微镜下切取28只Wistar雌性大鼠的两侧甲状旁腺,分别行组织和原代细胞培养。应用组织学形态学、PTH检测对甲状旁腺组织及原代培养细胞进行鉴定,并观察PTH的分泌规律。甲状旁腺组织及原代培养细胞组各分为正常磷、正常钙组(TN,CN,Pi1.0mml/l,Ca 1.25mmol/L),高磷、正常钙组(TH,CH,Pi3.5mmol/L,Ca 1.25mmol/L)。隔天换液,分别于培养0h、4h、12h、24h、48h、72h、5d、7d、9d留取培养上清液,检测iPTH。结果本实验切取的大鼠甲状旁腺组织和培养的甲状旁腺细胞经组织形态学及iPTH检测证实。在正常磷、正常钙的培养液中,甲状旁腺组织和培养的原代甲状旁腺细胞PTH分泌曲线相似,均在48h达到分泌高峰。在高磷、正常钙的培养液中甲状旁腺细胞PTH分泌与正常磷、正常钙组同一时间比较,差异无统计学意义(P〉0.05)。培养的甲状旁腺组织在高磷刺激下,PTH水平明显升高,最高达基础值的10倍以上(48h),除H0外,高磷、正常钙组(TH组)PTH与正常磷、正常钙组(TN组)同一时间比较差异均具有统计学意义(P〈0.001)。结论体外研究显示:高磷可以刺激甲状旁腺组织PTH分泌增加,而对分散的细胞无作用。进行高磷对甲状旁腺功能调节的体外研究,以选择甲状旁腺组织为宜,且时间选择在48h内。

关 键 词:高磷  甲状旁腺细胞原代培养  甲状旁腺组织  甲状旁腺激素
修稿时间:2008-04-11

The effect of phosphate on PTH release from parathyroid tissue and cultured cells in vitro
YANG Bing,WANG Mei. The effect of phosphate on PTH release from parathyroid tissue and cultured cells in vitro[J]. Chinese Journal of Blood Purification, 2008, 7(6): 317-320
Authors:YANG Bing  WANG Mei
Affiliation:( Department of Nephrology, Peking University People's Hospital, Beijing 100044, China)
Abstract:Objective To observe the levels of PTH secretion in primary cultured parathyroid cells and parathyroid tissues stimulated by high phosphate. Methods The parathyroid glands of 28 Wistar female rats were successfully resected by surgical operation under dissection microscope and were prepared for primary parathyroid cell culture and tissue culture. The cells and tissues were identified by histomorphology and PTH measurement and then cultured. Parathyroid tissues and cells were divided into 2 groups respectively as below: tissue groups: control group(TN, Pi 1.0mml/1, Ca 1.25mmol/1), experimental group (TH, Pi 3.5mmol/1, Ca 1.25mmol/1) cell groups: control group (CN, Pi 1.0mml/1, Ca 1.25mmol/1) and experimental group (CH, Pi 3.5mmol/1, Ca 1.25mmol/1).The medium was changed every two days. And the parathyroid hormone obtained from the supernatant for 0h,4h, 12h,24h, 48,72h,gd,7d,9d was measured. Results Parathyroid glands were identified by histomorphology and PTH measured. The PTH secretion curves of parathyroid cells and tissues incubated in normal phosphate and normal calcium medium were similar, and the peak time was 48h.Varing medium phosphate (1mM or 3.5mM) have no effect on PTH secretion (P 〉 0.05) in cell groups .But when PTG tissues were incubated in high phosphate medium, PTH levels could increase to 10-fold higher than baseline at 48h.There was a significant difference between group TH and group TN at the same time except H0 (P 〈 0.01).Conclusion High phosphate had a direct effect on PTH release from parathyroid tissue but not from dispersed parathyroid cells. Parathyroid tissues should be chosen to study the effect of phosphate on parathyroid function in vitro, and the time selected should be within 48h.
Keywords:High phosphate: Primary parathyroid cell culture  Parathyroid tissuc  Parathyroid hormone
本文献已被 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号