| 钙在嗜铬细胞瘤细胞缺氧性脑损伤中的作用研究 |
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| 引用本文： | 王新超,赵吉清,董兆君. 钙在嗜铬细胞瘤细胞缺氧性脑损伤中的作用研究[J]. 中国组织工程研究与临床康复, 2002, 6(15): 2217-2218 |
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| 作者姓名： | 王新超 赵吉清 董兆君 |
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| 作者单位： | 解放军第三军医大学预防医学系,重庆,400038 |
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| 摘 要： | 目的观察缺氧后大鼠肾上腺嗜铬细胞瘤(PC12)细胞内游离钙的浓度(犤Ca2+犦i)、环磷酸腺苷(cAMP)、钙调蛋白(CaM)和钙调蛋白激酶II(Ca2+/CaM-PKII)的变化。方法采用放免技术,从细胞水平进一步观察缺氧对PC12细胞的毒性、细胞内犤Ca2+犦i变化,cAMP、CaM和Ca2+/CaM-PKII的变化。结果缺氧组PC12细胞cAMP、CaM含量在缺氧后24h明显高于正常对照组;Ca2+/CaM-PKII活性明显低于正常对照组。结论犤Ca2+犦i、cAMP、CaM和Ca2+/CaM-PKII在缺氧PC12细胞损伤机制中起了重要的作用。
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| 关 键 词： | 缺氧 钙超载 钙调蛋白 环磷酸腺苷 钙调蛋白激酶 |
| 文章编号： | 1671-5926(2002)15-2217-02 |
| 修稿时间： | 2002-01-08 |
An experimental study on the effect o f Ca2+in PC12 after hypoxia injury |
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| WANG Xin-chao,ZHAO Ji-qing,DONG Zhao-jun,et al.. An experimental study on the effect o f Ca2+in PC12 after hypoxia injury[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2002, 6(15): 2217-2218 |
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| Authors: | WANG Xin-chao ZHAO Ji-qing DONG Zhao-jun et al. |
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| Affiliation: | WANG Xin-chao,ZHAO Ji-qing,DONG Zhao-jun,et al.Department of Toxicology,Third Military Medical University,Chongqing 400038,China |
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| Abstract: | Objective To observe the changes of the content ofi,CaM ,cAMP and activity of Ca 2+ /CaM-PK II after hypoxia in-jury.Methods The changes of the content ofi ,CaM ,cAMP and activity of Ca 2+/CaM-PK II were studied in PC12 cell after hypoxia injury with RIA.Results The changes of the content ofi ,CaM ,cAMP were significantly higher at hypoxia than at control group at hypoxia;but the activity of Ca 2+ /CaM-PK II were significantly decre ased.Conclusioni,CaM ,cAMP and Ca 2+ /CaM-PK II lead to important role in the damage in PC 12 after hypoxia injury. |
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| Keywords: | hypoxia Ca 2+ overload CaM cAMP Ca 2+ /CaM-PK II |
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