促红细胞生成素在子宫内膜异位症组织的表达

目的探讨促红细胞生成素(Epo)在子宫内膜异位症(EMs)发生中的病理生理作用及其临床意义。方法应用免疫组织化学SABC法、mRNA原位杂交法检测39例子宫内膜异位症的异位内膜及35例正常子宫内膜Epo的组织定位和表达强度,并对比其差异。结果Epo、EpomRNA的表达主要定位于腺体细胞胞质、胞核,间质细胞表达不明显。异位内膜组织Epo、EpomRNA表达分别为9.68±2.90,3.08±0.48,明显高于正常对照的4.54±2.86,0.55±0.46,差异有高度显著性(P<0.01);Epo、EpoRNA在EMs组、正常对照组的增殖期和分泌期表达相比,差异均无显著性(P>0.05);EMs组EpomRNA与AFS分期呈正相关(r=0.979,P=0.013)。结论Epo通过旁分泌或自分泌方式过度转录表达引起的局部新生血管生成增强可能是子宫内膜异位症发生的机制之一。

Expression of erythropoietin in endometriosis tissues

Objective:To investigate the expression of erythropoietin(Epo)in endometriosis and its correlation with clinical biological behaviors.Methods:Endometrium specimens were obtained from ectopic endometrium in 38 cases with endometriosis and 35 normal uterus for control.Expressions of Epo and its mRNA in these paraffin embraced specimens were examined by immunohistochemistry and in situ hybridization technique with specific cDNA probes.Results:Both expressions of Epo and Epo mRNA in glandular cells in endometrium of endometriosis were stronger than those in normal endometrium.Interstitial cell showed an insignificant expression.Expressions of Epo and Epo mRNA in endometriosis were 9.68±2.90 and 3.08±0.48,respectively,significantly higher than those with normal endometriosis(P<0.001). Expression of Epo mRNA was closely correlate with American Fertility Society endometriosis′ staging(r=0.979,P=0.013).Conclusion:The increased expressions of Epo and Epo mRNA in endometriosis may play important roles in the pathogenesis of endometriosis.