Voltage-gated sodium channel expression in rat spiral ganglion neurons |
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| Affiliation: | 1. Department of Cell Physiology and Pharmacology, University of Leicester, University Road, Leicester, LE1 9HN, UK;2. Neurosciences Centre of Excellence for Drug Discovery, GlaxoSmithKline Research & Development, New Frontiers Science Park, Harlow, Essex, CM19 5AW, UK;1. Janssen Research & Development, LLC, Titusville, NJ, USA;2. MAC Clinical Research, Manchester, UK;3. Analgesic Solutions, Natick, MA, USA;4. Tufts University School of Medicine, Boston, MA, USA;5. Massachusetts General Hospital & Harvard Medical School, Department of Neurology, Nerve Injury Unit, Boston, MA, USA;6. St Pancras Clinical Research, London, UK;1. Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705 USA;2. Department of Brain and Cognitive Sciences, Meliora Hall, University of Rochester, Rochester, NY 14627, USA;3. Department of Neuroscience, University of Rochester Medical Center, Rochester, NY 14642, USA;4. Department of Otolaryngology, University of Rochester Medical Center, Rochester, NY 14642, USA;1. Center for Hearing and Balance and Department of Otolaryngology-HNS, Johns Hopkins University, Baltimore, MD 21205, USA;2. Hearing Research Unit, Garvan Institute of Medical Research, Darlinghurst 2010, NSW, Australia;3. School of Medical Sciences, University of New South Wales, Kensington 2052, NSW, Australia;1. Universidade de São Paulo (USP), Faculdade de Medicina Ribeirão Preto (FMRP), Departamento de Oftalmologia e Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, Ribeirão Preto, SP, Brazil;2. Universidade de São Paulo (USP), Faculdade de Medicina Ribeirão Preto (FMRP), Departamento de Fisiologia, Ribeirão Preto, SP, Brazil |
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| Abstract: | The spiral ganglion neurons (SGN) provide the afferent innervation of the hair cells in the organ of Corti and relay auditory information from the inner ear to the brain. Voltage-gated sodium channels (NaV) initiate and propagate action potentials that encode this sensory information but little is known regarding the subtypes expressed in these cells. We have used RT-PCR and immunohistochemistry to study the compliment and anatomical distribution of NaV channels in rodent SGN. NaV1.1, NaV1.6 and NaV1.7 were all detected at the mRNA level. Fluorescence or streptavidin–horseradish peroxidase immunohistochemistry extended these findings, demonstrating predominant localisation of NaV1.6 and NaV1.7 on SGN cell bodies and NaV1.1 on axonal processes. Dual labelling with peripherin demonstrated higher NaV1.6 and NaV1.7 expression on Type I rather than Type II neurons. These results provide evidence for selective expression and variations in the distribution of VGSC in the rodent SGN, which may guide further studies into afferent function in the auditory pathway and therapeutic approaches for diseases such as hearing loss and tinnitus. |
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