硒、锌对梭曼诱导大鼠过氧化损伤的保护机制研究

目的 :　研究梭曼 (Soman)对大鼠急性中毒的自由基损伤作用及硒、锌的抗氧化保护作用。方法 :　雄性大鼠 40只 ,按体重随机分为阴性组 (正常对照组 )、阳性组 (损伤对照组 )、硒组 (加硒保护组 )及锌组 (加锌保护组 )。测定指标有血清、大脑、肝脏的维生素 E(VE)、总抗氧化能力 (T- AOC)及一氧化氮合成酶 (NOS)。结果 :　梭曼中毒后 ,T- AOC水平、VE含量明显下降 ,NOS活性显著提高 ;硒、锌组较阳性对照组损伤指标变化幅度较小。结果 :　梭曼中毒有明显的自由基损伤 ,硒、锌对梭曼中毒有显著的抗氧化作用。

STUDY ON THE PROTECTIVE EFFECTS OF SELENIUM AND ZINC AGAINST OXIDATIVE DAMAGE INDUCED BY SOMAN IN RATS

Objective: To study the effect of free radical injury induced by Soman in rats and protection by Se/Zn. [WT5FZ]Methods: [WT5BZ]40 male rats were randomly divided into 4 groups, i.e., negative group, positive group, Se group and Zn group according to weights. The serum, cerebrum and liver VE content, nitrogen oxide synthase (NOS activity and T AOC (total antioxidative capacity) were determined. Results: The VE content and T AOC decreased markedly, and NOS activities increased significantly after Soman intoxication. The changes were less significant in Se/Zn supplemented group. Conclusion: The mechanism of free radical injury is indicated in Soman intoxication and Se/Zn supplementation has significant protection.