氨基葡萄糖对体外培养人软骨和滑膜细胞软骨寡聚基质蛋白分泌影响的比较

目的 比较氨基葡萄糖对体外培养软骨和滑膜细胞软骨寡聚基质蛋白(COMP)分泌的影响.方法 软骨细胞和滑膜细胞采自骨关节炎(OA)患者,采用分阶段酶消化法体外培养人软骨细胞和滑膜细胞,给予实验兔以氨基葡萄糖临床等效剂量灌胃后,抽取含药血清培养细胞.采用酶联免疫吸附试验(ELISA)检测药物对体外培养的人软骨细胞和滑膜细胞培养上清液中COMP的浓度.结果 体外培养滑膜细胞分泌的COMP浓度[(41.6±0.4)ng/ml]显著高于软骨细胞[(5.5±3.0)ng/ml](19＜0.05).氨基葡萄糖可显著增加体外培养软骨细胞的COMP分泌[(20.3±3.6)与(5.5±3.0)ng/ml,P＜0.05];而对体外培养滑膜细胞的作用较弱,可轻度减少COMP分泌[(36.6±1.3)与(41.6±0.4)ng/ml].结论 氨基葡萄糖含药血清可以促进体外培养软骨细胞分泌COMP,而对体外培养滑膜细胞无明显作用.

Comparison of the effect of glucosamine on the cartilage oligomeric matrix protein secretion in vitro chondrocytes and synoviocytes

Objective To compare the effect of glucosamine (Virtral-s) on the cartilage oligomeric matrix protein (COMP) secretion of chondrocytes and synoviocytes in vitro.Methods Chondrocytes and synoviocytes isolated from knee cartilage of osteoarthritic patients were cultured by phased enzymatic digestion.Sera containing Virtral-s of the experimental animals were obtained after orally administrated Virtral-s at the dosages that equal to human.Cells were cultured in the medium with Virtral-s containing sera.Super-natant COMP level was tested by enzyme-linked immunoabsorbent assays (ELISA).Results COMP conceu-tration of synoviocytes cultured in vitro was significantly higher than that of chondrocytes (P＜0.05).Virtral-s could significantly increase COMP secretion in cultured chondrocytes in vitro (P＜0.05),however,it had a weaker role on synoviocytes,ie,it could only mildly reduce COMP secretion of synoviocytes.Conclusion Glucosamine (Virtral-s)-containing serum can promote COMP secretion of chondrocytes in vitro,and it has no significant effect on synoviocytes in vitro.