| 丹参山楂有效组分配伍抗动脉粥样硬化的实验研究 |
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| 引用本文： | 张建永,晏仁义,王岚,王建露,王万丹,侯瑞,刘红亮,杨洪军,梁日欣,杨滨.丹参山楂有效组分配伍抗动脉粥样硬化的实验研究[J].中国中药杂志,2013,38(12):1987-1991. |
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| 作者姓名： | 张建永 晏仁义 王岚 王建露 王万丹 侯瑞 刘红亮 杨洪军 梁日欣 杨滨 |
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| 作者单位： | 中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药研究所, 北京 100700;首都医科大学 中医药学院, 北京 100069;首都医科大学 中医药学院, 北京 100069;中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药研究所, 北京 100700 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09308-005,2012ZX09201201-003);山东省2012年科学技术发展计划(第三批2010GSF30025) |
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| 摘 要： | 目的: 探讨丹参山楂有效组分不同配伍抗动脉粥样硬化(atherosclerosis,AS)的作用,以期获得较优组合. 方法: 采用高脂饲料喂养,腹腔注射维生素D3及炎症诱导的方法建立AS大鼠模型.对丹参水溶性组分(DSA)、山楂水溶性组分(SZA)及丹参脂溶性组分(DSL),以3个剂量水平(大-中-小,1-2-3)进行正交实验,观察不同配伍对AS大鼠血脂水平、氧化应激、内皮功能及炎症反应的影响.采用主成分分析、聚类分析对试验结果进行多目标优化. 结果: 与模型组相比,各配伍样品均显示了降脂、抗氧化、改善内皮功能及抗炎等作用,方差分析表明DSA2-SZA2-DSL1,DSA3-SZA2-DSL1,DSA3-SZA3-DSL3, DSA3-SZA1-DSL1分别为降脂、抗氧化、内皮保护、抗炎作用的较优组合;多目标优化结果显示DSA2-SZA1-DSL2是抗AS的较优组合. 结论: 丹参山楂有效组分不同配伍均可在一定程度上干预大鼠AS的进程,不同配伍作用方式的侧重不同.
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| 关 键 词： | 丹参 山楂 有效组分配伍 动脉粥样硬化 主成分分析 |
| 收稿时间： | 2012/12/10 0:00:00 |
Experimental study on anti-atherosclerotic effect of compatibility of active components of Danshen and Shanzha |
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| ZHANG Jian-yong,YAN Ren-yi,WANG Lan,WANG Jian-lu,WANG Wan-dan,HOU Rui,LIU Hong-liang,YANG Hong-jun,LIANG Ri-xin and YANG Bin.Experimental study on anti-atherosclerotic effect of compatibility of active components of Danshen and Shanzha[J].China Journal of Chinese Materia Medica,2013,38(12):1987-1991. |
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| Authors: | ZHANG Jian-yong YAN Ren-yi WANG Lan WANG Jian-lu WANG Wan-dan HOU Rui LIU Hong-liang YANG Hong-jun LIANG Ri-xin and YANG Bin |
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| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;School of Chinese Medicine, Capital Medical University, Beijing 100069, China;School of Chinese Medicine, Capital Medical University, Beijing 100069, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | Objective: To investigate the effect of active components of Danshen and Shanzha of different matching proportions on atherosclerosis (AS), in the expectation of obtaining the optimum combination method. Method: Atherosclerotic rats were fed with high fat diet, and injected with vitamin D3 and ovalbumin. Aqueous extracts of Danshen (DSA) and Shanzha (SZA) and lipophilic extracts of danshen (DSL) were adopted for a low, medium and high-dose orthogonal experiment, to observe the effect of their different matching proportions on lipid level, oxidative stress, endothelial function and inflammatory reaction. The principal component analysis and cluster analysis were adopted for the multi-objective optimization of experimental results. Result: Compared with the model group, all of samples with different proportions of DSA, DSL and SZA showed effect in lowering lipid level, scavenging free radicals, reducing endothelial dysfunction and inhibiting inflammation. According to the variance analysis, DSA2-SZA2-DSL1, DSA3-SZA2-DSL1, DSA3-SZA3-DSL3 and DSA3-SZA1-DSL1 were the optimal proportions for lowering lipid level, scavenging free radicals, reducing endothelial dysfunction and inhibiting inflammation, respectively. According to the results of the multi-objective optimization, DSA2-SZA1-DSL2 was the optimal proportions of anti-AS. Conclusion: All of active components of Danshen and Shanzha of different matching proportions show the anti-AS effect in rats to varying degrees, but with different focus in different matching proportions. |
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| Keywords: | Danshen Shanzha proportion atherosclerosis principal component analysis |
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