| 雌激素效应元件调控的酵母报告载体的构建 |
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| 引用本文： | 何世华,梁增辉,战威,王福玉,贾凌志,晁福寰. 雌激素效应元件调控的酵母报告载体的构建[J]. 环境与健康杂志, 2003, 20(1): 39-41 |
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| 作者姓名： | 何世华 梁增辉 战威 王福玉 贾凌志 晁福寰 |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所,天津,300050 |
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| 基金项目： | 国家自然科学基金重点资助项目(30030120) |
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| 摘 要： | 目的：为建立环境雌激素基因重组酵母测评系统，构建雌激素效应元件（estrogen responseelements，ERE）调控的酵母报告载体。方法：人工合成ERE寡核苷酸片段，先与经SalI酶切和CIAP去磷酸化处理的pLacZi连接成pLacZi-1ERE，再与经XhoI酶切和CIAP去磷酸化处理的pLacZi-1ERE连接，得到pLacZi-2ERE。结果：序列分析结果表明，构建成功2个串联拷贝ERE调控的酵母报告载体。结论：该文构建成功ERE调控的酵母报告载体，为进一步建立环境雌激素基因重组酵母测评系统奠定了基础。
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| 关 键 词： | 酵母报告载体 环境雌激素 雌激素效应元件 酵母 报告基因 |
| 文章编号： | 1001-5914(2003)01-0039-03 |
| 修稿时间： | 2002-08-01 |
Construction of the Reporter Vector Controlled by Two Tandem Copies of Estrogen Response Elements in Yeast |
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| HE Shi-hua,LIANG Zeng-hui,ZHAN Wei,et al.. Construction of the Reporter Vector Controlled by Two Tandem Copies of Estrogen Response Elements in Yeast[J]. Journal of Environment and Health, 2003, 20(1): 39-41 |
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| Authors: | HE Shi-hua LIANG Zeng-hui ZHAN Wei et al. |
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| Affiliation: | HE Shi-hua,LIANG Zeng-hui,ZHAN Wei,et al.Institute of Health and Environmental Medicine of AMMS,Tianjin300050,Chi na |
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| Abstract: | Objective To establish the recombinant yeast estrogen system(RYES),a reporter vector controlled by estro-gen response elements(ERE)in yeast was constructed.Methods pLacZi-1RER was obtained first by inserting an ERE which was synthesized artificially into the yeast reporter vector pLacZi which had already been lined by SalI and the terminal dephos-phorized by CIAP.Then,pLacZi-zERE.was obtained by inserting another ERE into the XhoI site nearby the ERE in pLacZi-1ERE.Results Sequence analysis indicated that the yeast reporter vector controlled by two tandem copies of ERE was con-structed successfully.Conclusion The yeast reporter vector controlled by ERE,pLacZi-2ERE,was constructed successfully,which was the very important groundwork for further establishment of the recombinant yeast estrogen system(RYES). |
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| Keywords: | Environmental estrogens Estrogen response elements Yeast Reporter gene |
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