糖尿病大鼠视网膜中NGF的动态表达及巴曲酶对其的影响

目的探讨神经生长因子（NGF）在糖尿病大鼠视网膜中的动态变化规律，初步证实巴曲酶对NGF的影响并探讨其机制，为临床上治疗糖尿病视网膜病变（DR）提供理论依据。方法将大鼠腹腔注射链脲佐菌素制作实验性糖尿病模型，再分别于成模后2个月和3个月时腹腔注射巴曲酶进行干预。通过免疫组织化学和原位杂交双重检测NGF在视网膜中的表达。结果糖尿病（DM）造模后2个月时视网膜中NGF的含量明显减少，且随时间变化有统计学差异，而巴曲酶治疗后NGF的表达明显增加。结论DR大鼠视网膜中NGF表达减少可能参与DR的发病机制。巴曲酶对DR有保护作用，其机制可能包括对NGF的调节。

The Dynamic Expression of NGF and the Impact of Batroxobin on it in the Retina of Diabetic Rats

Objective To investigate the dynamic regularity of nerve growth factor in the retina of diabetic rats and to confirm the impact of batroxobin on NGF tentatively and the mechanism in order to furnish the theory foundation for the therapy of DR clinically. Methods Through the peritoneal injection of streptozocin in rats to make the experimental diabetes models, we ejected batroxobin in abdominal cavity of rats differently 2 months and 3 months after the formation of DM model for intervention. Through the immunohistochemistry and hybridization in situ to detect the expression of NGF in retina of the diabetic rats.Results The content of NGF in retina of the diabetic rats decreased obviously 2 months after the formation of DM model and it had time-varying statistics diversity, however the expression of NGF was markedly increased after the treatment of batroxobin, Conclusion The decreased expression of NGF in retina of DR rats might play an important role in the pathogenesis of DR.Treatment with batroxobin is beneficial in the prevention of DR, possibly through the regulation of the expression level of NGF in retina of the DR rats.