| 胰岛素对家兔血浆支、芳氨基酸浓度与其比值的影响 |
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| 引用本文： | 魏涌,徐蘅,汤秀玉,王征宇,周霞,彭长青. 胰岛素对家兔血浆支、芳氨基酸浓度与其比值的影响[J]. 南京医科大学学报(自然科学版), 1989, 0(2) |
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| 作者姓名： | 魏涌 徐蘅 汤秀玉 王征宇 周霞 彭长青 |
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| 作者单位： | 南京铁道医学院生化教研室(魏涌，徐蘅，汤秀玉，王征宇)，南京铁道医学院消化科(周霞)，南京铁道医学院消化科(彭长青) |
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| 摘 要： | 给饥饿家免按每公斤体重4u于皮下注射药用胰岛素。注射前、后30分钟,分别抽血并分离出血浆。用聚酰胺薄层析分离和荧光测试法检测血浆支链氨基酸(BCAA)及芳香族氨基酸(AAA)荧光强度,并以此计算支/芳(BCAA/AAA)比值。以对照液用同样方法做对照组实验。研究结果,AAA变化不显著,但BCAA则明显降低,因而支/芳比值从注射前的2.816下降至注射后的1.486,其下降百分率为48.0%。提示,增加血浆胰岛素的有效浓度能显著改变BCAA的分布平衡。病例测试,选择具有高胰岛素血症的肝硬化患者20例及肝性脑病患者13例,以同样方法进行血浆支/方比值测定,所获结果与上述动物实验的相互吻合。
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| 关 键 词： | 胰岛素 氨基酸 支链 芳香族氨基酸 支/芳比值 色谱法 薄层 透视定位法 兔 |
THE INFLUENCE OF INSULIN ON THE CONCENTRATIONS OF BCAA AND AAA, RATIO IN THE PLASMA OF RABBITS |
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| Wei Yong. THE INFLUENCE OF INSULIN ON THE CONCENTRATIONS OF BCAA AND AAA, RATIO IN THE PLASMA OF RABBITS[J]. Acta Universitatis Medicinalis Nanjing, 1989, 0(2) |
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| Authors: | Wei Yong |
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| Abstract: | The concentrations of the BCAA (V. I. L) and AAA (F, Y) were determined and themolar ratio of BCAA/AAA calculated in the plasma of fasting rabbits 30 minutes afterhypodermic insulin (4 I. U./kg. body(?)wt.). Strongly fluorescent dansyl amino acids bythin-layer microchromatography were identified and measured. The experimental groupshowed an increase in plasma BCAA whereas the change in AAA concentrations was nil.The calculated BCAA/AAA ratio decreased from 2.816 to 1.486 after the injection. Theresults suggested that an effective dose of insulin caused a change in the distributionequilibrium of the plasma BCAA. Plasma BCAA/AAA in patients with liver cirrhosis (20 cases) and chronic hepato-encephalopathy (13 cases) showed similar changes. |
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| Keywords: | insulin amino acids branched-chain aromatic amino acids hyperinsulinism chromagraphy thin layer fluorometey rabbits |
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