| 利用噬菌体表面展示技术克隆抗人DR5抗体可变区基因 |
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| 引用本文： | 刘瑞敏,王明丽,季泽俊,刘峰涛,白慧玲,马远方. 利用噬菌体表面展示技术克隆抗人DR5抗体可变区基因[J]. 河南大学学报(医学版), 2011, 30(1): 51-53 |
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| 作者姓名： | 刘瑞敏 王明丽 季泽俊 刘峰涛 白慧玲 马远方 |
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| 作者单位： | 河南大学细胞与免疫学重点实验室,河南,开封,475004 |
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| 基金项目： | 传染病预防控制国家重点实验室开放课题，河南省重点科技攻关资助项目 |
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| 摘 要： | 目的:利用噬菌体表面展示技术制备抗人DR5单链抗体.方法:从抗DR5杂交瘤细胞中提取总RNA,RT-PCR扩增VH和VL基因,并利用重叠扩增PCR将VH和VL拼接为scFv基因.将scFv与PAK100载体通过相同的酶切位点连接后,转化大肠杆菌XL1-Blue,经VSCM13辅助噬菌体拯救,构建成鼠抗人DR5单链抗体库...
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| 关 键 词： | 噬菌体展示技术 单链抗体 死亡受体5 |
Clone of the anti-DR5 antibody variable sequence by phage surface display |
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| LIU Rui-min,WANG Ming-li,JI Ze-jun,LIU Feng-tao,BAI Hui-ling,Ma Yuan-fang. Clone of the anti-DR5 antibody variable sequence by phage surface display[J]. Journal of Henan University, 2011, 30(1): 51-53 |
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| Authors: | LIU Rui-min WANG Ming-li JI Ze-jun LIU Feng-tao BAI Hui-ling Ma Yuan-fang |
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| Affiliation: | LIU Rui-min,WANG Ming-li,JI Ze-jun,LIU Feng-tao,BAI Hui-ling,Ma Yuan-fang(Key Laboratory of Cellular and Molecular Immunology,Henan University,Kaifeng,Henan 475000,China) |
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| Abstract: | Objective:To obtain the gene sequence of antibody against human death receptor 5 by the phage surface display.Methods:By the technique of phage display,the heavy chain and light chain variable region genes(VH and VL) were assembled into single chain antibodies(scFv) with overlap extension reaction by L inker primer mix.The scFv were cloned into PAK100 phagemid and introduced into E.coli XL1-Blue.The phagemid containing bacterial colonies were infected with VSCM13,then the total surface display library of an... |
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| Keywords: | Phage surface display technique Single chain antibodies Death receptor 5 |
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