三种方法治疗大鼠布比卡因系统毒性的效果比较

目的 观察三种方法对布比卡因系统毒性的治疗效果,探寻局麻药毒性安全有效的治疗方法.方法 36只雄性SD大鼠随机分为丙泊酚组(P组)、咪唑安定组(M组)和丙泊酚+咪唑安定组(PM组),每组12只.以肢体Ⅱ导联监测心电图(electrocardiogram, ECG),股动脉置入24G套管针监测血压,股静脉置入24G套管针...

Therapeutic Effects of Three Methods on Bupivacaine-induced Systemic Toxicity in Rats

Objective To compare the therapeutic effects of three methods on bupivacaine-induced toxicities in rats, and explore a safe and effective treatment method on the toxicities of local anesthetic. Methods Thirty-six male SD rats were randomly assigned to three groups （n=12 each）： propofol group （group P）, midazolam group （group M） and propofol and midazolam group （group PM）. Lead Ⅱ of electrocardiogram （ECG） was continuously monitored. The femoral artery was cannulated for direct measurement of arterial blood pressure. The femoral vein was cannulated for infusion of 0.5% bupivacaine at the rate of 2 mg/（kg·min） until mean arterial blood pressure （MAP） decreased to 5.3 kPa or less. And then within 30 seconds, group P was infused of propofol 50 mg/kg, group M of midazolam 5 mg/kg and group PM of propofol 25 mg/kg and midazolam 2.5mg/kg. The infused volume was 5ml/kg. Results There were no significant differences among three groups in the occurrence time of toxicities reaction including arrhythmias, convulsion and MAP≤5.3kPa, and the total dosage of bupivacaine. MAP of group PM was significantly higher than that of group P at 1, 3 and 5 minutes, and higher than that of group M at 3 and 5 minutes after resuscitation treatment （P0.05）. The survival rate was significantly increased in group PM （P0.05）. Conclusion Propofol combined with midazolam has a synergistic therapeutic efficacy on severe bupivacaine-induced toxicities in rats, and can reduce the side effects of drugs and improve the survival rate of rats.